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Biotin Mouse Anti-Human Eotaxin
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG2b, κ
Synthetic human eotaxin
ELISA Detection (Routinely Tested), Western blot (Reported)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

ELISA Detector: The biotinylated monoclonal antibody 10C11 (Cat. No. 555060) is useful as a detection antibody in a sandwich ELISA for measuring human eotaxin protein levels. Biotinylated monoclonal antibody 10C11 can be paired with the purified monoclonal antibody 3C7 (Cat. No. 555035) with recombinant human eotaxin (Cat. No. 555102) as the standard. Biotinylated monoclonal antibody 10C11 should be titrated to determine its optimal concentration for ELISA detector (0.5 - 2.0 µg/ml). To obtain linear standard curves, doubling dilutions of recombinant human eotaxin (Cat. No. 555102) ranging from ~1000 to 20 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site,

Note 1: This ELISA pair shows no cross-reactivity with any of the chemokines tested (human MCP-1, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β mouse Eotaxin, MCP-1; and rat MCP-1).

Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. For assay of serum or plasma samples, the specially formulated BD OptEIA™ Human Eotaxin ELISA Set, Cat. No. 555175 is recommended.

Western Blot: The purified 10C11 antibody has been reported to be useful for Western Blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
555060 Rev. 1
Antibody Details
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The monoclonal antibody 10C11 reacts with human chemokine eotaxin. The immunogen used to generate the monoclonal antibody 10C11 was synthetic human eotaxin.

This antibody is routinely tested by ELISA detection. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

555060 Rev. 1
Format Details
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Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
555060 Rev.1
Citations & References
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Development References (1)

  1. Ponath PD, Qin S, Ringler DJ, et al. Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils. J Clin Invest. 1996; 97(3):604-612. (Clone-specific). View Reference
555060 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.