BD™ Cytometric Bead Array (CBA) Human Total IgG Flex Set
Description
The BD™ CBA Human Total IgG Flex Set is a bead-based immunoassay capable of measuring human total immunoglobulin G (IgG) in serum and plasma samples. Human reactivity was determined by testing samples with the BD CBA Human Total IgG Flex Set. The biology and function of IgG has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Immunoglobulin Master Buffer Kit (Cat. No. 558683).
Preparation And Storage
This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and when reconstituted in 1.0 ml Assay Diluent has a total IgG protein concentration of 13,760 ng/ml. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect PE Detection Reagent from prolonged exposure to light.
Recommended Assay Procedures
The BD CBA Human Total IgG Flex Set must be used in conjunction with a BD CBA Human Immunoglobulin Master Buffer Kit (Cat. No. 558683, 100 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Immunoglobulin Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human Total IgG Flex Set will be 430 ng/ml. An example standard curve is shown in Figure 1.
Due to the levels of immunoglobulin protein in typical samples and specific assay conditions, the BD CBA Human Total IgG Flex Set should not be used in the same assay well with any other BD CBA Flex Set assay. It should only be run as a single-plex assay. For an updated assay compatibility chart for the BD CBA Human Immunoglobulin Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/flexset.
Performance
Limit of Detection: The theoretical limit of detection is 0.34 ng/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/ml, n=30) + 2 standard deviations.
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.