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      Z-FA-FMK, Negative Control for Caspase Inhibitors
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      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #

      BD Pharmingen™ Z-FA-FMK, Negative Control for Caspase Inhibitors

      (RUO)
      Z-FA-FMK, Negative Control for Caspase Inhibitors
      Flow cytometric analysis of apoptosis in Jurkat cells (Human T-cell leukemia; ATCC TIB-152).  Jurkat cells were preincubated with the following: no inhibitor (upper left and bottom left panels), 20 µM Z-LEHD-FMK (a caspase-9 inhibitor) (upper center and bottom center panels) or 20 µM Z-FA-FMK negative control inhibitor (upper right and bottom right panels) for 30 minutes, and then either left untreated (bottom row) or treated with 4 µM campthothecin for 3 hours (top row). Following incubation, cells were collected and stained with PE Annexin V (Cat. No. 559763) to identify cells undergoing apoptosis. The results indicate that in campthothecin treated cells, approximately 42% of the cells were induced to undergo apoptosis and the use of the negative control inhibitor, Z-FA-FMK, showed similar results to the treated cells without inhibitor (right panels), indicating that the negative control inhibitor did not attenuate apoptosis.
      Z-FA-FMK, Negative Control for Caspase Inhibitors
      Flow cytometric analysis of apoptosis in Jurkat cells (Human T-cell leukemia; ATCC TIB-152).  Jurkat cells were preincubated with the following: no inhibitor (upper left and bottom left panels), 20 µM Z-LEHD-FMK (a caspase-9 inhibitor) (upper center and bottom center panels) or 20 µM Z-FA-FMK negative control inhibitor (upper right and bottom right panels) for 30 minutes, and then either left untreated (bottom row) or treated with 4 µM campthothecin for 3 hours (top row). Following incubation, cells were collected and stained with PE Annexin V (Cat. No. 559763) to identify cells undergoing apoptosis. The results indicate that in campthothecin treated cells, approximately 42% of the cells were induced to undergo apoptosis and the use of the negative control inhibitor, Z-FA-FMK, showed similar results to the treated cells without inhibitor (right panels), indicating that the negative control inhibitor did not attenuate apoptosis.
      Product Details
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      Description

      Members of the caspase family play key roles in inflammation and mammalian apoptosis. Z-FA-FMK is a negative control inhibitor which has no inhibitory effect on apoptosis mediated by caspases and can only inhibit cysteine proteases (those not requiring P1 Asp inhibitors) such as Cathepsin B. Z-FA-FMK has a molecular weight of 386 Daltons.



      Preparation And Storage

      Avoid multiple freeze-thaws of product.

      Store lyophilized Z-FA-FMK at -20˚C. Reconstitute Z-FA-FMK in DMSO before use. Reconstituted Z-FA-FMK may be stored in small aliquots at -20˚C.

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      Recommended Assay Procedures

      Z-FA-FMK is designed to be used in both in vivo and in vitro cell based assays as a negative control in assays used to measure apoptosis. Reconstitute 1.0 mg of Z-FA-FMK in DMSO. A 10 mM stock solution may be made by dissolving 1.0 mg of Z-FA-FMK in 263 µl DMSO. The final concentration of Z-FA-FMK needed may vary between experimental systems and investigators are encouraged to titrate. As a precautionary note, please do not exceed a final DMSO concentration of 0.2% as higher levels may cause cellular toxicity and mask the effects of the caspase inhibitor.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      550411 Rev. 4
      Citations & References
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      Development References (2)

      1. Gregoli PA, Bondurant MC. Function of caspases in regulating apoptosis caused by erythropoietin deprivation in erythroid progenitors. J Cell Physiol. 1999; 178(2):133-143. (Biology). View Reference
      2. Thornberry NA, Lazebnik Y. Caspases: enemies within. Science. 1998; 281(5381):1312-1316. (Biology). View Reference
      550411 Rev. 4

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.