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Multiparameter flow cytometric analysis of TCR Cβ2 expression on Human peripheral blood lymphocytes. Human whole blood was stained with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; Left Plot), BD Horizon™ R718 Mouse Anti-Human TCR Cβ2 antibody (Cat. No. 571544/571586; Middle and Right Plots) and BD OptiBuild™ RB545 Mouse Anti-Human TCR Cβ1 antibody (Cat. No. 756564; Right Plot) at 0.5 µg/test. Erythrocytes were lysed with BD FACS Lysing™ Solution (Cat. No. 349202). The pseudocolor density plots showing the correlated expression of TCR Cβ2 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals or TCR Cβ1 were derived from gated events with the light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony™ A5 SE Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
BD Horizon™ R718 Mouse Anti-Human TCR Cβ2
Regulatory Status Legend
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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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Companion Products
The SAM.2.rMAb is a recombinant monoclonal antibody that recognizes TCR Cβ2 expressed by a large proportion of CD4+ and CD8+ T cells. Thymocytes and mature peripheral T cells predominantly express a heterodimeric T cell receptor (TCR αβ) for antigen which is comprised of disulfide-liked transmembrane α and β chain subunits. The constant region of the TCR α subunit is encoded by TRAC, whereas the TCR β subunit is encoded by either of two highly homologous constant region genes, TCRB1 for TCR Cβ1 or TCRB2 for TCR Cβ2. The JOVI.1 antibody alternatively recognizes TCR Cβ1 expressed by the other TCR αβ+ T cells. These antibodies are effectively used together in multicolor staining and flow cytometric analyses to identify and characterize the natures of either TCR Cβ1+ or TCR Cβ2+ T cells in heterogeneous cell populations.
Note - Some human CD3- and TCR-specific antibodies might not be compatible to co-stain human T cells with SAM2.rMab. To confirm compatibility and immunofluorescent staining protocols, please visit: https://www.bdbiosciences.com/content/dam/bdb/marketing-documents/discover-learn/thought-leadership/events/POSTER-AAI%20202024-S-Riguad.pdf
Development References (7)
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Berg H, Otteson GE, Corley H, et al. Flow cytometric evaluation of TRBC1 expression in tissue specimens and body fluids is a novel and specific method for assessment of T-cell clonality and diagnosis of T-cell neoplasms.. Cytometry B Clin Cytom. 2021; 100(3):361-369. (Biology). View Reference
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Ferrari M, Baldan V, Ghongane P, et al. Targeting TRBC1 and 2 for the treatment of T cell lymphomas. Abstract. Cancer Res. 2020; 80:2183. (Biology).
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Horna P, Shi M, Olteanu H, Johansson U. Emerging Role of T-cell Receptor Constant β Chain-1 (TRBC1) Expression in the Flow Cytometric Diagnosis of T-cell Malignancies.. Int J Mol Sci. 2021; 22(4):1817. (Biology). View Reference
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Maciocia PM, Wawrzyniecka PA, Philip B, et al. Targeting the T cell receptor β-chain constant region for immunotherapy of T cell malignancies.. Nat Med. 2017; 23(12):1416-1423. (Biology). View Reference
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Morath A, Schamel WW. αβ and γδ T cell receptors: Similar but different.. J Leukoc Biol. 2020; 107(6):1045-1055. (Biology). View Reference
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Muñoz-García N, Lima M, Villamor N, et al. Anti-TRBC1 Antibody-Based Flow Cytometric Detection of T-Cell Clonality: Standardization of Sample Preparation and Diagnostic Implementation.. Cancers (Basel). 2021; 13(17):4379. (Biology). View Reference
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Tesio M. Subtle Differences to Make the Difference.. Hemasphere. 2018; 2(2):e38. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.