The SY/11B5 monoclonal antibody specifically binds to CD157 which is also known as BST-1 (Bone marrow stromal antigen 1), BP-3/IF-7, Mo5, ADP-ribosyl cyclase 2, and cADPr hydrolase 2. CD157 is a 40-46 kDa glycophosphatidylinositol-linked cell membrane glycoprotein. It is an ectoenzyme that has both cyclic ADP-ribose hydrolase and ADP-ribosyl cyclase activities. CD157 is expressed as a homodimer by a variety of cell types including bone marrow stromal cells, granulocytes, monocytes, macrophages, dendritic cells, endothelial cells, and B and T cell progenitors. In addition to its ectoenzyme activities, CD157 reportedly functions as a receptor involved in neutrophil and monocyte adhesion, transendothelial migration and diapedesis and in tumor cell migration.
The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.