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Purified Rat Anti-Mouse CD140a
Product Details
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BD Pharmingen™
Pdgfra; Pdgfr2; PDGF-R alpha; Platelet derived growth factor receptor alpha
Mouse (QC Testing)
Rat WF, also known as Wistar Furth IgG2a, κ
Mouse PDGF Receptor α chain
Flow cytometry (Routinely Tested), ELISA, Functional assay, Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Western blot (Reported)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

This antibody has been tested by immunofluorescent staining (≤ 1 µg/million cells) with flow cytometric analysis to assure specificity and reactivity. Other reported applications include western blot analysis, ELISA, immunohistochemical staining of paraffin-embedded sections, inhibition of ligand binding, and in vivo blocking of PDGF action, but these were not tested at BD.

Product Notices

  1. Please refer to for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to to access safety data sheets (SDS).
558774 Rev. 8
Antibody Details
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The APA5 antibody monoclonal antibody specifically binds to CD140a, the Platelet-Derived Growth Factor Receptor alpha chain (PDGFR-a, PDGFR-α). CD140a is a receptor tyrosine kinase that is widely expressed on cells of mesenchymal origin in the embryo and adult. CD140a is expressed on several other cell types during embryonic development, but not on hematopoietic cells. CD140a binds to PDGF A and B chains, in contrast to PDGFR-b (CD140b) that binds only to the PDGF B chain.  Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms. Ligand binding to the PDGF Receptor induces the formation of receptor dimers (aa, ab, or bb), autophosphorylation, and internalization.  The APA5 antibody has been demonstrated to block binding of  PDGF-AA to PDGFR-a-expressing cells in vitro  and to block some PDGF-mediated developmental events in vivo.

558774 Rev. 8
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
558774 Rev.8
Citations & References
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View product citations for antibody "558774" on CiteAb

Development References (3)

  1. Fruttiger M, Calver AR, Krüger WH. PDGF mediates a neuron-astrocyte interaction in the developing retina. Neuron. 1996; 17(6):1117-1131. (Clone-specific: Blocking). View Reference
  2. Heldin CH. Structural and functional studies on platelet-derived growth factor. EMBO J. 1992; 11(12):4251-4259. (Biology). View Reference
  3. Takakura N, Yoshida H, Kunisada T, Nishikawa S, Nishikawa SI. Involvement of platelet-derived growth factor receptor-alpha in hair canal formation. J Invest Dermatol. 1996; 107(5):770-777. (Immunogen: Blocking, ELISA, Immunohistochemistry, Inhibition, Western blot). View Reference
558774 Rev. 8

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.