Purified NA/LE Rat Anti-Mouse CD102

BD Pharmingen™ Purified NA/LE Rat Anti-Mouse CD102

Name: Purified NA/LE Rat Anti-Mouse CD102
Brand: Purified NA/LE Rat Anti-Mouse CD102
SKU: 553325

Clone 3C4(mIC2/4) (RUO)

Product Details

Brand: BD Pharmingen™

Alternative Name: ICAM-2

Reactivity: Mouse (QC Testing)

Isotype: Rat LEW, also known as Lewis IgG2a, κ

Immunogen: Transfected Cell Line

Application: Flow cytometry (Routinely Tested), Blocking, Immunohistochemistry-frozen, Immunoprecipitation (Reported), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)

Concentration: 1.0 mg/ml

Entrez Gene ID: 15896

RRID: AB_394783

Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Purified NA/LE Rat IgG2a, κ Isotype Control RUO

Size 0.5 mg Cat No. 553926

FITC Goat Anti-Rat Ig RUO

Size 0.5 mg Cat No. 554016

553325 Rev. 11

Antibody Details

3C4(mIC2/4)

The 3C4 (mIC2/4) monoclonal antibody specifically binds to the mouse ICAM-2 (CD102) cell surface glycoprotein, a ligand for LFA-1. CD102 is constitutively expressed on endothelial cells, T and B lymphocytes, and alveolar walls. It is also expressed on a variety of leukocyte cell lines. CD102 does not appear to be involved in the development of hematopoietic cells. In a model for allergic asthma, endothelial CD102 mediates the transmigration of eosinophils (but not lymphocytes, monocytes, or macrophages) into the airway lumen. The 3C4 (mIC2/4) antibody blocks interactions between ICAM-2 and LFA-1.

553325 Rev. 11

Format Details

NA/LE

NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

Format: NA/LE

553325 Rev.11

Citations & References

View product citations for antibody "553325" on CiteAb

Development References (2)

Gerwin N, Gonzalo JA, Lloyd C, et al. Prolonged eosinophil accumulation in allergic lung interstitium of ICAM-2 deficient mice results in extended hyperresponsiveness. Immunity. 1999; 10(1):9-19. (Biology: Immunohistochemistry). View Reference
Xu H, Bickford JK, Luther E, Carpenito C, Takei F, Springer TA. Characterization of murine intercellular adhesion molecule-2. J Immunol. 1996; 156(12):4909-4914. (Immunogen: Blocking, Immunoprecipitation). View Reference

553325 Rev. 11

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.