Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Companion Products
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The RPM.7 monoclonal antibody specifically recognizes CD9, a 26-kDa member of the transmembrane 4 superfamily. Rat CD9 mRNA is detected in many adult tissues, including neural and hematopoietic tissue. Using RPM.7 mAb in flow cytometric analysis of rat hematopoietic tissues, CD9 is found on platelets, peripheral B- and T-lymphocyte subsets, and leukocyte subpopulations in the bone marrow. In humans, CD9 has been found to be associated with integrins and other cell-surface receptors, and it is suggested to play a role in signal transduction and possibly in regulating cellular adhesive properties. In mice, CD9 has been demonstrated to participate in T-cell costimulation and induction of apoptosis.
Development References (6)
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Claas C, Wahl J, Orlicky DJ, et al. The tetraspanin D6.1A and its molecular partners on rat carcinoma cells. Biochem J. 2005; 389(Pt 1):99-110. (Clone-specific: Immunoprecipitation). View Reference
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Jennings LK, Crossno JT Jr, Fox CF, White MM, Green CA. Platelet p24/CD9, a member of the tetraspanin family of proteins. Ann N Y Acad Sci. 1994; 714:175-184. (Biology). View Reference
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Kaprielian Z, Cho KO, Hadjiargyrou M, Patterson PH. CD9, a major platelet cell surface glycoprotein, is a ROCA antigen and is expressed in the nervous system. J Neurosci. 1995; 15(1):562-573. (Biology). View Reference
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Park CS, Yashiro Y, Tai XG, et al. Differential involvement of a Fas-CPP32-like protease pathway in apoptosis of TCR/CD9-costimulated, naive T cells and TCR-restimulated, activated T cells.. J Immunol. 1998; 160(12):5790-6. (Biology). View Reference
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Terada N, Baracskay K, Kinter M, et al. The tetraspanin protein, CD9, is expressed by progenitor cells committed to oligodendrogenesis and is linked to beta1 integrin, CD81, and Tspan-2. Glia. 2002; 40(3):350-359. (Clone-specific: Immunoprecipitation). View Reference
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Wright MD, Tomlinson MG. The ins and outs of the transmembrane 4 superfamily. Immunol Today. 1994; 15(12):588-594. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.