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      Purified Mouse Anti-NHP CD45
      Purified Mouse Anti-NHP CD45
      Western Blot analysis of CD45 expression on peripheral blood of Rhesus macaque (Macaca mulatta). Rhesus blood was stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 556648; lanes 1 and 4), Purified Mouse Anti-NHP CD45 (clone D058-1283; Cat. No. 552566; lanes 2 and 5), or Purified Mouse Anti-Human CD45 (clone TÜ116; lanes 3 and 6). Samples either non-reduced and shown at 1 second exposure (lanes 1-3), or reduced and shown at 10 seconds exposure (lanes 4-6). Staining was visualized with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988).
      Purified Mouse Anti-NHP CD45
      Flow cytometric analysis of CD45 expression on peripheral blood of Rhesus macaque (Macaca mulatta). Rhesus blood was stained with Purified Mouse Anti-NHP CD45 (clone D058-1283), followed by FITC Goat Anti-Mouse IgG/IgM. The dot plot depicting side scatter versus CD45 expression was derived from gated events with the side and forward light-scattering characteristics of viable cells.
      Purified Mouse Anti-NHP CD45 Purified Mouse Anti-NHP CD45
      Western Blot analysis of CD45 expression on peripheral blood of Rhesus macaque (Macaca mulatta). Rhesus blood was stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 556648; lanes 1 and 4), Purified Mouse Anti-NHP CD45 (clone D058-1283; Cat. No. 552566; lanes 2 and 5), or Purified Mouse Anti-Human CD45 (clone TÜ116; lanes 3 and 6). Samples either non-reduced and shown at 1 second exposure (lanes 1-3), or reduced and shown at 10 seconds exposure (lanes 4-6). Staining was visualized with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988).
      Flow cytometric analysis of CD45 expression on peripheral blood of Rhesus macaque (Macaca mulatta). Rhesus blood was stained with Purified Mouse Anti-NHP CD45 (clone D058-1283), followed by FITC Goat Anti-Mouse IgG/IgM. The dot plot depicting side scatter versus CD45 expression was derived from gated events with the side and forward light-scattering characteristics of viable cells.
      Product Details
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      BD Pharmingen™
      Pan Leukocyte, NHP-specific; PTPRC; LCA; L-CA; Leukocyte Common Ag
      Rhesus, Cynomolgus, Baboon (QC Testing)
      Mouse IgG1, κ
      Rhesus peripheral whole blood
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_394433
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      552566 Rev. 4
      Antibody Details
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      D058-1283

      D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes. It was developed using Rhesus peripheral whole blood as the immunogen. It does not cross-react with human leucocytes. This antibody reacts with baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern to CD45 binding to leukocyte common antigen (LCA) on human cells. Immunophenotypic analysis shows that D058-1283 binds to lymphocytes, monocytes and granulocytes of non-human primate blood samples. This antibody is able to block the binding of monoclonal antibody TÜ116; a reported anti-human CD45 antibody that cross-reacts with nonhuman primate leucocytes. In Western blot analysis, the D058-1283 antibody identifies a 180-200 kDa band.

      552566 Rev. 4
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      552566 Rev.4
      Citations & References
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      View product citations for antibody "552566" on CiteAb

      Development References (3)

      1. Kishihara K, Penninger J, Wallace VA, et al. Normal B lymphocyte development but impaired T cell maturation in CD45-exon6 protein tyrosine phosphatase-deficient mice. Cell. 1993; 74(1):143-156. (Biology). View Reference
      2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
      3. Reimann KA, Waite BC, Lee-Parritz DE, et al. Use of human leukocyte-specific monoclonal antibodies for clinically immunophenotyping lymphocytes of rhesus monkeys. Cytometry. 1994; 17(1):102-108. (Biology). View Reference
      552566 Rev. 4

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.