Skip to main content Skip to navigation
Hamburger Menu
Search icon
Location Selector Location Selector
India (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Close Menu

If you notice text formatting issues, please update your browser to view the website correctly

See More

Close
Website Feedback
Alert Icon
38521A_551900_image1.png

Flow cytometric analysis of CD32 expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram) or Purified Mouse Anti-Human CD32 (Cat. No. 551900; solid line histogram). Secondary staining was carried out with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988) and erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescent histograms were derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes.

38521A_551900_image1.png Blue-Cap-White-Label.jpg

Flow cytometric analysis of CD32 expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram) or Purified Mouse Anti-Human CD32 (Cat. No. 551900; solid line histogram). Secondary staining was carried out with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988) and erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescent histograms were derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
FcγRII; CD32a/FcγRIIa/FCGR2A; CD32b/FcγRIIb/FCGR2B
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_394290
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Show More blue down arrow Show Less blue up arrow

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Show More blue down arrow Show Less blue up arrow
551900 Rev. 4
Antibody Details
Down Arrow Up Arrow
3D3

The 3D3 monoclonal antibody specifically recognizes FcγRII (CD32), a 40 kDa, polymorphic type I transmembrane glycoprotein that serves as a low affinity receptor for aggregated IgG. This highly glycosylated molecule (encoded by at least two different genes) is expressed on monocytes, granulocytes, platelets and B cells. Unlike the FLI28.26 mAb, the 3D3 mAb detected a polymorphic CD32 antigen expressed on B cells of all donors, but only on platelets, monocytes and granulocytes of some donors. The platelets from 3D3+ donors respond to certain stimulatory mAb such as CD165 (clone SN2) which results in aggregation. On the other hand, the platelets from 3D3 negative donors do not form aggregates after stimulation. Individuals can be divided into two groups as responder and non-responder depending on expression, or non-expression, of 3D3. In comparison to the 3D3 mAb, the FLI8.26 mAb detects a monomorphic CD32 antigen expressed on all human donors.

551900 Rev. 4
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
551900 Rev.4
Citations & References
Down Arrow Up Arrow
View product citations for antibody "551900" on CiteAb

Development References (2)

  1. Gosselin EJ, Brown MF, Anderson CL, Zipf TF, Guyre PM. The monoclonal antibody 41H16 detects the Leu 4 responder form of human Fc gamma RII. J Immunol. 1990; 144(5):1817-1822. (Biology). View Reference
  2. Vely F, Gruel N, Moncuit J, et al. A new set of monoclonal antibodies against human Fc gamma RII (CD32) and Fc gamma RIII (CD16): characterization and use in various assays.. Hybridoma. 1997; 16(6):519-28. (Immunogen). View Reference
551900 Rev. 4

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.