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Purified Mouse anti-Human CD110
Purified Mouse anti-Human CD110

Western blot analysis of human CD110 (Thrombopoietin receptor) expressed by human HEL92.1.7 cells and platelets. Lysates prepared from human HEL92.1.7 cells and platelets were SDS-PAGE electrophoresed and transferred to membranes. They were then probed using Purified Mouse Anti-Human CD110 antibody (Cat. No. 562137) at concentrations of 0.25 (lane 1), 0.125 (lane 2) and 0.06 (lane 3) μg/ml. CD110 is identified as approximately 65-72 kDa bands from HEL cells (Left Panel) and 80-90 kDa bands from platelets (Right Panel). The molecular masses observed for the Thrombopoietin Receptor protein using the 1.78.1 antibody for Western blotting may vary due to splice variations and/or different post-translational modifications of the receptor obtained from different cell types.

Western blot analysis of human CD110 (Thrombopoietin receptor) expressed by human HEL92.1.7 cells and platelets. Lysates prepared from human HEL92.1.7 cells and platelets were SDS-PAGE electrophoresed and transferred to membranes. They were then probed using Purified Mouse Anti-Human CD110 antibody (Cat. No. 562137) at concentrations of 0.25 (lane 1), 0.125 (lane 2) and 0.06 (lane 3) μg/ml. CD110 is identified as approximately 65-72 kDa bands from HEL cells (Left Panel) and 80-90 kDa bands from platelets (Right Panel). The molecular masses observed for the Thrombopoietin Receptor protein using the 1.78.1 antibody for Western blotting may vary due to splice variations and/or different post-translational modifications of the receptor obtained from different cell types.

Product Details
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BD Pharmingen™
C-MPL; MPL; MPLV; TPOR; Thrombopoietin receptor
Human (QC Testing)
Mouse IgG1, κ
Human c-Mpl extracellular domain
Western blot (Routinely Tested), Flow cytometry (Tested During Development)
65–72 kDa (HEL cells) and 80–90 kDa (Platelets)
0.5 mg/ml
AB_10894391
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
562137 Rev. 2
Antibody Details
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1.78.1

The 1.78.1 monoclonal antibody specifically binds to the human Thrombopoietin Receptor (TPO-R) that is also know as the Myeloproliferative leukemia protein (c-Mpl) or CD110. CD110 is a type I transmembrane protein and a member of the hematopoietin receptor family. It is expressed on hematopoietic stem cells, a subfraction of hematopoietic precursor cells, cells of the megakaryocytic lineage and platelets. CD110 serves as a receptor for thrombopoietin. Upon binding of thrombopoietin to CD110, megakaryocyte proliferation and differentiation is induced and stem cells are protected from apoptosis.

562137 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
562137 Rev.2
Citations & References
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Development References (4)

  1. Abbott C, Huang G, Ellison AR, et al. Mouse monoclonal antibodies against human c-Mpl and characterization for flow cytometry applications.. Hybridoma (Larchmt). 2010; 29(2):103-13. (Clone-specific: Flow cytometry). View Reference
  2. Broudy VC, Lin NL, Fox N, Taga T, Saito M, Kaushansky K. Thrombopoietin stimulates colony-forming unit-megakaryocyte proliferation and megakaryocyte maturation independently of cytokines that signal through the gp130 receptor subunit. Blood. 1996; 88(6):2026-2032. (Biology). View Reference
  3. Deng B, Banu N, Malloy B, et al. An agonist murine monoclonal antibody to the human c-Mpl receptor stimulates megakaryocytopoiesis. Blood. 1998; 92(6):1981-1988. (Biology). View Reference
  4. Gotoh A, Ritchie A, Takahira H, Broxmeyer HE. Thrombopoietin and erythropoietin activate inside-out signaling of integrin and enhance adhesion to immobilized fibronectin in human growth-factor-dependent hematopoietic cells. Ann Hematol. 1997; 75(5-6):207-213. (Biology). View Reference
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562137 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.