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PE Rat anti-Mouse CD125
PE Rat anti-Mouse CD125

Flow cytometric analysis of PE-conjugated anti-mouse CD125 on mouse bone marrow cells. Isolated murine bone marrow cells were stained with either PE anti-CD125 (clone T21, Cat. No. 558488, right panel) or a PE-conjugated rat IgG1 isotype control (Cat. No. 557078, left panel) and FITC anti-CD11b (clone M1/70, Cat. No. 553310).  Flow cytometry was performed on a BD FACSCalibur™ System and the histograms were derived from the gated events based on light scattering characteristics of viable bone marrow cells.

Flow cytometric analysis of PE-conjugated anti-mouse CD125 on mouse bone marrow cells. Isolated murine bone marrow cells were stained with either PE anti-CD125 (clone T21, Cat. No. 558488, right panel) or a PE-conjugated rat IgG1 isotype control (Cat. No. 557078, left panel) and FITC anti-CD11b (clone M1/70, Cat. No. 553310).  Flow cytometry was performed on a BD FACSCalibur™ System and the histograms were derived from the gated events based on light scattering characteristics of viable bone marrow cells.

Product Details
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BD Pharmingen™
Il5r; Il5ra; IL-5RA; IL-5Rα; IL-5R-alpha; IL-5R subunit alpha
Mouse (QC Testing)
Rat WI, also known as Wistar (outbred) IgG1, λ
Mouse T88-M Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_647186
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Recommended Assay Procedures

For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that the cells be pre-incubated with Mouse BD Fc Block™ Purified anti-Mouse CD16/CD32 mAb, Clone 2.4G2 (Cat. No. 553141/553142).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558488 Rev. 1
Antibody Details
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T21

The T21 monoclonal antibody specifically binds to CD125 which is also known as the alpha subunit of the IL-5 Receptor (IL5RA/IL-5Rα).  CD125 is a type I transmembrane protein that belongs to the hemopoietin receptor and Ig gene superfamilies. It is expressed on B cells and eosinophils. CD125 associates with the Common β chain (βc/Bc) subunit, also known as CD131, to form the functional IL-5 Receptor complex. Mouse IL-5 belongs to the hematopoietic growth factor family of cytokines and promotes the growth and differentiation of B cells and eosinophils. Upon binding, the T21 antibody blocks IL-5 binding to its receptor and can inhibit IL-5 induced B-cell proliferation and antibody production.

558488 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
558488 Rev.1
Citations & References
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Development References (3)

  1. Adachi T, Alam R.. The mechanism of IL-5 signal transduction. Am J Physiol. 275(3 Pt 1):C623-C633. (Biology). View Reference
  2. Hitoshi Y, Yamaguchi N, Mita S, et al. Distribution of IL-5 receptor-positive cells. Expression of IL-5 receptor in Ly-1 (CD5)+ B cells.. J Immunol. 1990; 144(11):4218-4225. (Biology). View Reference
  3. Wetzel, GD. Interleukin 5 regulation of peritoneal Ly-1 B lymphocyte proliferation, differentiation, and autoantibody secretion. Eur J Immunol. 1989; 19(9):1701-1707. (Biology). View Reference
558488 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.