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PE Mouse Anti-Human Notch3
PE Mouse Anti-Human Notch3

Flow cytometric analysis of Notch3 expression on human Acute Lymphoblastic Leukemia cell line. Human MOLT-3 cells (ATCC CRL-1552™) were harvested and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 556650; dashed line histogram) or PE Anti-Human Notch3 antibody (solid line histogram) at matched concentrations.  Fluorescence histograms were derived from gated events with the forward and side-light scattering characteristics of viable MOLT-3 cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometry System.

Flow cytometric analysis of Notch3 expression on human Acute Lymphoblastic Leukemia cell line. Human MOLT-3 cells (ATCC CRL-1552™) were harvested and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 556650; dashed line histogram) or PE Anti-Human Notch3 antibody (solid line histogram) at matched concentrations.  Fluorescence histograms were derived from gated events with the forward and side-light scattering characteristics of viable MOLT-3 cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometry System.

Product Details
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BD Pharmingen™
NOTCH3; CADASIL; CASIL; Neurogenic locus notch homolog protein 3
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human Notch3 Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
4854
AB_2738300
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. All other brands are trademarks of their respective owners.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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Antibody Details
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MHN3-21

The MHN3-21 monoclonal antibody specifically binds to an extracellular domain of human Notch3. Notch3 is a type 1 transmembrane glycoprotein receptor and member of the Notch family that includes Notch1-Notch4. The Notch3 precursor is cleaved in the Golgi and presents as a cell surface heterodimeric receptor. The Notch3 receptor can bind to several membrane-bound ligands including Jagged1, Jagged2 and Delta1. Upon ligand binding, Notch3 undergoes proteolytic cleavage that results in the release of the Notch intracellular domain, NICD. NICD translocates to the nucleus where it can form a transcriptional activator complex with various transcription factors. These multimeric complexes either positively or negatively regulate the expression of multiple genes including those that orchestrate many facets of embryonic development and the subsequent functioning of organ systems such as the hematopoietic, immune, nervous and cardiovascular systems. Notch3 is reportedly expressed by monocytes but not by CD34+ cells and most types of normal lymphocytes. Notch3 has also been implicated in the development of T cell neoplasia. Notch3 is expressed in vascular smooth muscle cells and plays a key role in neural development.  Mutations in Notch3 have been identified as the underlying cause of CADASIL, a cerebral autosomal dominant disease that is the most common form of hereditary stroke disorder.

  

563593 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
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Citations & References
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Development References (3)

  1. Haraguchi K, Suzuki T, Koyama N et al. Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL-15. J Immunol. 2009; 182(10):6168-6178. (Immunogen: Blocking). View Reference
  2. Joutel A, Corpechot C, Ducros A, et al. Notch3 mutations in CADASIL, a hereditary adult-onset condition causing stroke and dementia. Nature. 1996; 383(6602):707-710. (Biology). View Reference
  3. Pancewicz J, Nicot C. Current views on the role of Notch signaling and the pathogenesis of human leukemia. BMC Cancer. 2011; 11:502-508. (Biology). View Reference
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Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.