PE Mouse anti-Human Notch2

BD Pharmingen™ PE Mouse anti-Human Notch2

Name: PE Mouse anti-Human Notch2
Brand: BD Pharmingen™
SKU: 563519

Clone MHN2-25

(RUO)

Flow cytometric analysis of Notch2 expression on human MCF-7 cells. Human MCF7 cells (ATCC Cat. No. HTB-22™; breast adenocarcinoma cell line) were harvested and stained with either PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648; dashed line histogram) or PE Anti-Human Notch2 antibody (Cat. No. 563519; solid line histogram) at matched concentrations. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable MCF-7 cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometry System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: NOTC2; AGS2; hN2; HJCYS; Neurogenic locus notch homolog protein 2

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG2a, κ

Immunogen: Human Notch2 Recombinant Protein

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

RRID: AB_2738251

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

PE Mouse IgG2a, κ Isotype Control RUO

Size 0.1 mg Cat No. 554648

563519 Rev. 1

Antibody Details

MHN2-25

The MHN2-25 monoclonal antibody specifically binds to an extracellular domain of human Notch2. Notch2 is a type 1 transmembrane glycoprotein receptor and member of the Notch family that includes Notch1-Notch4. Notch2 is cleaved in the Golgi and presents as a cell surface heterodimeric receptor. The Notch2 receptor can bind to several membrane-bound ligands including Jagged1, Jagged2, Delta1 and Delta4. Upon ligand binding, Notch2 undergoes proteolytic cleavage that results in the release of the Notch intracellular domain, NICD. NICD translocates to the nucleus where it forms a transcriptional activator complex with various transcriptional factors. These multimeric complexes either positively or negatively regulate the expression of multiple genes including those that orchestrate many facets of embryonic development and the subsequent functioning of multiple organ systems such as the hematopoietic, immune, cardiovascular, hepatic and renal systems. Notch2 is expressed by cells of the lung and brain, as well as cells of the B lineage, thymocytes, and T cells. Abnormalities in Notch2 expression may play a role in the development of B cell lymphomas.

563519 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

563519 Rev.1

Citations & References

Development References (4)

Chiba S. Notch signaling in stem cell systems. Stem Cells. 2006; 24(11):2437-2447. (Biology). View Reference
Haraguchi K, Suzuki T, Koyama N et al. Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL-15. J Immunol. 2009; 182(10):6168-6178. (Immunogen: Flow cytometry). View Reference
Lammert E, Brown J, Melton DA. Notch gene expression during pancreatic organogenesis. 2000; 94(1-2):199-203. (Biology). View Reference
Lee SY, Kumano K, Nakazaki K et al. Gain-of-function mutations and copy number increases of Notch2 in diffuse large B-cell lymphoma. 2009; 100(5):920-926. (Immunogen: ELISA). View Reference

View All (4)

563519 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.