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PE Mouse anti-Human HLA-ABC
PE Mouse anti-Human HLA-ABC

Flow cytometric analysis of PE anti-human HLA-A,B,C on lymphocytes. Whole blood was stained with PE anti-human HLA-A,B,C  (clone DX17 , Cat. No. 560168) and compared to whole blood stained with a PE mouse IgG1 isotype control (clone MOPC-21, Cat. No. 555749).   The isotype control is represented by a dashed line and the PE anti-human HLA-A,B,C by the solid line. Flow cytometry was performed on a BD FACSCalibur™ System and the histograms were derived from the gated events based on light scattering characteristics of viable cells.

Flow cytometric analysis of PE anti-human HLA-A,B,C on lymphocytes. Whole blood was stained with PE anti-human HLA-A,B,C  (clone DX17 , Cat. No. 560168) and compared to whole blood stained with a PE mouse IgG1 isotype control (clone MOPC-21, Cat. No. 555749).   The isotype control is represented by a dashed line and the PE anti-human HLA-A,B,C by the solid line. Flow cytometry was performed on a BD FACSCalibur™ System and the histograms were derived from the gated events based on light scattering characteristics of viable cells.

Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Polyclonal Human NK Cell Line
Flow cytometry (Routinely Tested)
20 µl
AB_1645510
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
560168 Rev. 1
Antibody Details
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DX17

The DX17 monoclonal antibody reacts with a monomorphic epitope expressed on all HLA (human leukocyte antigen) class I molecules examined.  DX17 immunoprecipitates HLA class I heavy chains (45 kDa) and β2-microglobulin (12kDa) from radiolabeled human cell lines.  HLA is determined by a complex segment of the short arm of chromosome 6 and there are many human HLAs encoded in this segment. The antigenic agglomerate is called MHC, for major histocompatibility complex. Examples of class I loci are HLA-A, -B, and -C, which are serologically assayed; class II loci, e.g., HLA-D/DR and DC1, are tested by lymphocytotoxic methods.

560168 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
560168 Rev.1
Citations & References
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Development References (4)

  1. Bach FH, Amos DB. Hu-1: Major histocompatibility locus in man. Science. 1967; 156(781):1506-1508. (Biology). View Reference
  2. Gumperz JE, Litwin V, Phillips JH, Lanier LL, Parham P. The Bw4 public epitope of HLA-B molecules confers reactivity with natural killer cell clones that express NKB1, a putative HLA receptor. J Exp Med. 1995; 181(3):1133-1144. (Biology). View Reference
  3. Lanier LL, Gumperz JE, Parham P, Melero I, López-Botet M, Phillips JH. The NKB1 and HP-3E4 NK cells receptors are structurally distinct glycoproteins and independently recognize polymorphic HLA-B and HLA-C molecules. J Immunol. 1995; 154(7):3320-3327. (Clone-specific). View Reference
  4. Morton CC, Kirsch IR, Nance WE, Evans GA, Korman AJ, Strominger JL. Orientation of loci within the human major histocompatibility complex by chromosomal in situ hybridization. Proc Natl Acad Sci U S A. 1984; 81(9):2816-2820. (Biology). View Reference
View All (4) View Less
560168 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.