Flow cytometric analysis of Galectin-9 expression on human MOLT-4 cells. Cells from the human MOLT-4 (T lymphoblastic leukemia, ATCC CRL-1582) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were then stained with PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human Galectin-9 antibody (Cat. No. 565890/565892; solid line histogram). The fluorescence histogram showing Galectin-9 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System.
Multiparameter flow cytometric analysis of Galectin-9 expression on human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells (PBMC) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were then stained with PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human Galectin-9 antibody (Cat. No. 565890/565892; Right Plot). Two-parameter flow cytometric contour plots showing the correlated expression of Galectin-9 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
.png){kind=spacer}
Flow cytometric analysis of Galectin-9 expression on human MOLT-4 cells. Cells from the human MOLT-4 (T lymphoblastic leukemia, ATCC CRL-1582) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were then stained with PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human Galectin-9 antibody (Cat. No. 565890/565892; solid line histogram). The fluorescence histogram showing Galectin-9 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System.
Multiparameter flow cytometric analysis of Galectin-9 expression on human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells (PBMC) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were then stained with PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human Galectin-9 antibody (Cat. No. 565890/565892; Right Plot). Two-parameter flow cytometric contour plots showing the correlated expression of Galectin-9 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Product Details
BD Pharmingen™
galectin 9; Gal-9; Ecalectin; HUAT; LGALS9; LEG9; Tumor antigen HOM-HD-21
Human (QC Testing)
Mouse IgG1, κ
Human Galectin-9 (AA 208-215) Peptide
Intracellular staining (flow cytometry) (Routinely Tested), Flow cytometry (Reported)
5 µl
AB_2739384
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
Perm/Wash Buffer RUO
Size
100 mL
Cat No.
554723
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
PE Mouse Anti-Human Galectin-9 RUO
Size
100 Tests
Cat No.
565890
565892 Rev. 1
Antibody Details
9M1-3
The 9M1-3 monoclonal antibody specifically binds to Galectin-9 (Gal-9) which is encoded by LGALS9 (lectin, galactoside-binding, soluble, 9). Galectin-9 is differentially expressed within or on the surface of various cell types including lymphocytes, macrophages, astrocytes, mast cells, eosinophils, fibroblasts, and endothelial cells. Its expression can be induced by cells in response to proinflammatory cytokines or infection by certain viruses. Galectin-9 is an ~ 50 kDa S-type lectin that has two carbohydrate recognition domains (CRDs). It binds to β-galactosides and can serve as a ligand for TIM-3 (CD366). Cell-surface or released forms of Galectin-9 can play multiple roles in innate and adaptive immune responses including the activation of macrophage cytokine secretion, promotion of dendritic cell maturation and regulatory T cell expansion, and negative regulation of Th1, Th17, NK, and cytotoxic T cells. Galectin-9 may contribute to the survival and growth of some tumors by suppressing anti-tumor immune responses. The 9M1-3 antibody reportedly binds to the CRD2 domain of Galectin-9 and can block its binding to TIM-3.
`
565892 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565892 Rev.1
Citations & References
View product citations for antibody "565892" on CiteAb
Development References (7)
-
Anderson AC, Anderson DE. TIM-3 in autoimmunity.. Curr Opin Immunol. 2006; 18(6):665-9. (Biology). View Reference
-
Cedeno-Laurent F, Dimitroff CJ. Galectins and their ligands: negative regulators of anti-tumor immunity.. Glycoconj J. 2012; 29(8-9):619-25. (Biology). View Reference
-
Dai SY, Nakagawa R, Itoh A, et al. Galectin-9 induces maturation of human monocyte-derived dendritic cells.. J Immunol. 2005; 175(5):2974-81. (Biology). View Reference
-
Elahi S, Niki T, Hirashima M, Horton H. Galectin-9 binding to Tim-3 renders activated human CD4+ T cells less susceptible to HIV-1 infection.. Blood. 2012; 119(18):4192-204. (Clone-specific: Flow cytometry). View Reference
-
Gieseke F, Kruchen A, Tzaribachev N, Bentzien F, Dominici M, Müller I. Proinflammatory stimuli induce galectin-9 in human mesenchymal stromal cells to suppress T-cell proliferation.. Eur J Immunol. 2013; 43(10):2741-9. (Clone-specific: Flow cytometry). View Reference
-
Klibi J, Niki T, Riedel A, et al. Blood diffusion and Th1-suppressive effects of galectin-9-containing exosomes released by Epstein-Barr virus-infected nasopharyngeal carcinoma cells.. Blood. 2009; 113(9):1957-66. (Immunogen: Blocking, Flow cytometry, Functional assay, Inhibition). View Reference
-
Qiu Y, Chen J, Liao H, et al. Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions.. PLoS Pathog. 2012; 8(11):e1002984. (Clone-specific: Flow cytometry). View Reference
565892 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.