You are now leaving the BD Biosciences website. The site you are about to visit is operated by a third party. The link to this site neither makes nor implies any representation or warranty for any products or services offered on a third-party site and is intended only to enable convenient access to the third-party site and for no other purpose. Do you want to continue?
The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The polyclonal antibody solution was passed through solid-phase immunoadsorbent gels to minimize cross reactivity with rat, human, bovine, and horse serum proteins.
As a second-step reagent for staining rat spleen and bone marrow leukocytes, Cat. No. 550589 is reactive with mouse primary antibodies having the IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA isotypes. Minimal background staining of rat leukocytes occurs in the absence of primary antibody. In addition, it stains mouse peripheral B lymphocytes with little non-specific staining of other splenic leukocytes. Therefore, it is useful as a primary reagent in immunofluorescent staining of mouse B cells and antibody-producing cells and as a secondary reagent for staining rat leukocytes with mouse Ig primary antibodies. However, we have observed that the reactivity of polyclonal second-step antibodies to mouse or rat IgM may be reduced after adsorption against Ig of rat or mouse, respectively. Because this anti-mouse Ig antibody was adsorbed with rat Ig, it may be weakly with some mouse IgM primary antibodies. In those cases, we recommend anti-mouse IgM mAb R6-60.2 (Cat. No. 553409) or anti-mouse Ig κ light chain mAb 187.1 (Cat. No. 559940). For optimal staining of human leukocytes, with mouse IgG and IgM primary antibodies, we recommend FITC-conjugated goat anti-mouse IgG + IgM (human-, bovine-, and horse-adsorbed, Cat. No. 555988).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.