PE F(ab')2 Donkey anti-Rabbit IgG (Multiple Adsorption)

BD Phosflow™ PE F(ab')2 Donkey anti-Rabbit IgG (Multiple Adsorption)

Name: PE F(ab')2 Donkey anti-Rabbit IgG (Multiple Adsorption)
Brand: PE F(ab')2 Donkey anti-Rabbit IgG (Multiple Adsorption)
SKU: 558416

Clone poly/anti-rabbit IgG (RUO)

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Product Details

Brand: BD Phosflow™

Isotype: Donkey Ig

Immunogen: Rabbit IgG

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Vol. Per Test: 20 µl

RRID: AB_1645469

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

The polyclonal F(ab')2 antibody fragments were purified from donkey antiserum by pepsin digestion and immunoaffinity chromatography. The whole IgG and Fc fragments were removed, thereby reducing the Fc-mediated non-specific binding of this antibody to Fc receptor-bearing cells.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

558416 Rev. 3

Antibody Details

Donkey anti-rabbit IgG

The polyclonal F(ab')2 antibody reacts with heavy chains of rabbit IgG and light chains of most rabbit immunoglobulins, and not with non-immunoglobulin proteins of rabbit serum. It has minimal cross-reactivity with human, mouse, rat, bovine, caprine, equine, ovine, chicken, guinea pig, and Syrian hamster serum proteins.

558416 Rev. 3

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

558416 Rev.3

Citations & References

View product citations for antibody "558416" on CiteAb

558416 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

BD Phosflow™ PE F(ab')2 Donkey anti-Rabbit IgG (Multiple Adsorption)

Clone poly/anti-rabbit IgG (RUO)

Preparation And Storage

Product Notices

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