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      FITC Rat Anti-Mouse IgG3
      FITC Rat Anti-Mouse IgG3
      Flow cytometric analysis of intracellular mouse IgG3 on hybridoma cells. Permeabilized cells were stained with FITC Rat Anti-Mouse IgG3 (Cat. No. 553403; solid line histogram) or with FITC Rat IgG1, κ Isotype Control (Cat. No. 554688; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable hybridoma cells. Flow cytometry was performed on a FACSCalibur™.
      FITC Rat Anti-Mouse IgG3
      Flow cytometric analysis of intracellular mouse IgG3 on hybridoma cells. Permeabilized cells were stained with FITC Rat Anti-Mouse IgG3 (Cat. No. 553403; solid line histogram) or with FITC Rat IgG1, κ Isotype Control (Cat. No. 554688; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable hybridoma cells. Flow cytometry was performed on a FACSCalibur™.
      Product Details
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      BD Pharmingen™
      Ighg3; Immunoglobulin heavy constant gamma 3
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
      Mouse Pooled Ig
      Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)
      0.5 mg/ml
      AB_394840
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      Recommended Assay Procedures

      FITC Rat Anti-Mouse IgG3 may be used as a primary or secondary reagent in immunofluorescent staining. FITC Rat Anti-Mouse IgG3 is effective for detection of cell-surface or intracellular Ig by immunofluorescent staining with flow cytometric analysis. Note: intracellular staining is not routinely tested at BD Biosciences Pharmingen. The BD Cytofix/Cytoperm™ Fixation/Permeablization Kit, (Cat. No. 554714) contains the appropriate reagents for intracellular staining.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      553403 Rev. 14
      Antibody Details
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      R40-82

      The R40-82 antibody specifically recognizes mouse IgG3 of all strains. It does not react with other Ig isotypes.

      553403 Rev. 14
      Format Details
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      553403 Rev.14
      Citations & References
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      Development References (2)

      1. Duong BH, Ota T, Aït-Azzouzene D, et al. Peripheral B cell tolerance and function in transgenic mice expressing an IgD superantigen.. J Immunol. 2010; 184(8):4143-58. (Biology). View Reference
      2. Kuzin II, Snyder JE, Ugine GD, et al. Tetracyclines inhibit activated B cell function.. Int Immunol. 2001; 13(7):921-31. (Biology). View Reference
      553403 Rev. 14

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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