Dear Customer, If you experience any text readability or display issues on our website, please update your web browser to the latest version
See More
Product Details
BD OptiBuild™
SIGLEC6; CD33 antigen-like 1; CD33L1; CD33L; OBBP1; obesity-binding protein 1
Human (Tested in Development)
Mouse IgG2a, κ
Human Siglec-6 Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
AB_2872373
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV650 under optimal conditions that minimize unconjugated dye and antibody.
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 650 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
- Alexa Fluor® is a registered trademark of Life Technologies Corporation.
Companion Products
Human BD Fc Block™ RUO
Size
50 µg
Cat No.
564219
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Brilliant Stain Buffer RUO
Size
100 Tests
Cat No.
563794
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 ML
Cat No.
349202
747911 Rev. 1
Antibody Details
767329
The 767329 monoclonal antibody specifically recognizes Sialic acid-binding Ig-like lectin 6 (Siglec-6) which is also known as Obesity-binding protein 1 (OB-BP1), CD33 antigen-like 1 (CD33L1), or CD327. Siglec-6 (CD327) is a type I transmembrane glycoprotein that is encoded by SIGLEC6 which belongs to the Sialic acid-binding Ig-like lectins (Siglecs) superfamily. Siglec-6 (CD327) has one N-terminal Ig-like V-set domain followed by two Ig-like C2-type domains in its extracellular region. Its intracellular region has an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as a signaling lymphocyte activating molecule (SLAM)-like motif that can serve as a docking site for SAP (SLAM-associated protein). This I-type lectin can also exist in a soluble form of the extracellular region that lacks the transmembrane and cytoplasmic regions. Siglec-6 (CD327) is expressed on placental trophoblasts and B cells and at low levels on neutrophils. It specifically recognizes sialyl Tn-containing molecules and binds to leptin and is involved in cellular interactions and signaling.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
747911 Rev. 1
Format Details
BV650
The BD Horizon Brilliant Violet™ 650 (BV650) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 406-nm and an acceptor dye with an emission maximum (Em Max) at 649-nm. BV650, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 650-nm (e.g., a 660/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV650
406 nm
649 nm
747911 Rev.1
Citations & References
View product citations for antibody "747911" on CiteAb
Development References (3)
-
Botting RA, Bertram KM, Baharlou H, et al. Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes.. J Leukoc Biol. 2017; 101(6):1393-1403. (Clone-specific: Flow cytometry). View Reference
-
Madge PD, Maggioni A, Pascolutti M, et al. Structural characterisation of high affinity Siglec-2 (CD22) ligands in complex with whole Burkitt's lymphoma (BL) Daudi cells by NMR spectroscopy.. Sci Rep. 2016; 6:36012. (Clone-specific: Flow cytometry). View Reference
-
Takei Y, Sasaki S, Fujiwara T, Takahashi E, Muto T, Nakamura Y. Molecular cloning of a novel gene similar to myeloid antigen CD33 and its specific expression in placenta.. Cytogenet Cell Genet. 1997; 78(3-4):295-300. (Biology). View Reference
747911 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.