The MH7E7 monoclonal antibody specifically recognizes the extracellular domain of mouse Dendritic cell inhibitory receptor 4 (DCIR4). DCIR4 is a lectin receptor that is encoded by Clec4a1 (C-type lectin domain family 4, member a1) which belongs to the dendritic cell immunoreceptor (DCIR) family. DCIR4 (Clec4a1) is variably expressed on CD11b+ monocytes found in peripheral blood, secondary lymphoid tissues and the bone marrow. DCIR4 (Clec4a1) is reportedly expressed at low levels on Ly-6C-positive inflammatory monocytes and at high levels on Ly-6C-negative patrolling monocytes. It is not significantly expressed on eosinophils and neutrophils.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.