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      BV510 Rat Anti-Mouse CD326
      BV510 Rat Anti-Mouse CD326
      Multicolor analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142) and stained with BD Horizon™ BV510 Rat IgG2a, κ Isotype Control (Cat. No. 562952) or BD Horizon™ BV510 Rat Anti-Mouse CD326 antibody (Cat. No. 563216).    Left Panel: Thymocytes were further stained with FITC Rat Anti-Mouse CD4 (553047/553046/561835) and PE Rat Anti-Mouse CD8a (553033/553032/561095) antibodies. The CD326 (solid line) and Ig Isotype Control (dashed line) fluorescence histograms  were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes.    Middle and Right Panels: The splenic leucocytes were further stained with FITC Hamster Anti-Mouse CD3e (Cat. No 553062/553061/561827) and PE Rat Anti-Mouse CD25 (Cat. No. 553866/561065) antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining (Middle Plot) or CD326 (Right Plot) versus CD3e for CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected (Right Panel), whereas the CD25- T cells do not express detectable levels of CD326 (data not shown).    Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      BV510 Rat Anti-Mouse CD326
      Multicolor analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142) and stained with BD Horizon™ BV510 Rat IgG2a, κ Isotype Control (Cat. No. 562952) or BD Horizon™ BV510 Rat Anti-Mouse CD326 antibody (Cat. No. 563216).    Left Panel: Thymocytes were further stained with FITC Rat Anti-Mouse CD4 (553047/553046/561835) and PE Rat Anti-Mouse CD8a (553033/553032/561095) antibodies. The CD326 (solid line) and Ig Isotype Control (dashed line) fluorescence histograms  were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes.    Middle and Right Panels: The splenic leucocytes were further stained with FITC Hamster Anti-Mouse CD3e (Cat. No 553062/553061/561827) and PE Rat Anti-Mouse CD25 (Cat. No. 553866/561065) antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining (Middle Plot) or CD326 (Right Plot) versus CD3e for CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected (Right Panel), whereas the CD25- T cells do not express detectable levels of CD326 (data not shown).    Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      Product Details
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      BD Horizon™
      Ep-CAM; EGP; EGP-2; Egp314; GA733-2; TROP1; Tacsd1; Tacstd1; Ly74; gp40
      Mouse (QC Testing)
      Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ
      Glycoconjugates from BALB/c mouse-derived TE-71 medullary thymic epithelial cell line
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_2738075
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Brilliant Violet™ 510 is a trademark of Sirigen.
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      563216 Rev. 1
      Antibody Details
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      G8.8

      The G8.8 monoclonal antibody recognizes CD326/Ep-CAM (Epithelial Cell Adhesion Molecule), also known as gp40 in the mouse and by a variety of names (including GA733-2, CO17-1A, and EGP) in the human.  In the mouse, Ep-CAM is a 40-42 kDa cell-surface type 1 transmembrane glycoprotein expressed on thymic epithelial cells, thymic dendritic cells, immature thymocytes, a small subset of peripheral T lymphocytes, intestinal epithelium, kidney-collecting tubule epithelium, keratinocytes, Langerhans cells and lymph node and splenic dendritic cells. Profiles of Ep-CAM expression on fetal thymocytes and on the CD4[-] CD8[-], CD4[+] CD8[+], CD4[-] CD8[+], and CD4[+] CD8[-] subsets of adult thymocytes have been published.  In unrelated studies, mouse Ep-CAM mRNA was detected in tissues containing epithelial cells (kidney, stomach, intestine, lung, and thymus) and in plasma cells and plasmacytomas, but not in heart, muscle, liver, brain, spleen, B lymphomas, or pre-B lymphomas.  Ep-CAM is a Ca[2+] independent homophilic adhesion molecule that is proposed to play roles in the development and normal function of epithelial tissues and in the progression of carcinomas.

      The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

      563216 Rev. 1
      Format Details
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      BV510
      The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV510
      Violet 405 nm
      327 nm, 405 nm
      512 nm
      563216 Rev.1
      Citations & References
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      View product citations for antibody "563216" on CiteAb

      Development References (9)

      1. Basak S, Speicher D, Eck S, Wunner W. Colorectal carcinoma invasion inhibition by CO17-1A/GA733 antigen and its murine homologue. J Natl Cancer Inst. 1998; 90(9):691-697. (Biology). View Reference
      2. Bergsagel PL, Victor-Kobrin C, Timblin CR, Trepel J, Kuehl WM. A murine cDNA encodes a pan-epithelial glycoprotein that is also expressed on plasma cells. J Immunol. 1992; 148(2):590-596. (Biology). View Reference
      3. Birebent B, Somasundaram R, Purev E. Anti-idiotypic antibody and recombinant antigen vaccines in colorectal cancer patients. Crit Rev Oncol Hematol. 2001; 39((1-2)):107-113. (Biology). View Reference
      4. Borkowski TA, Nelson AJ, Farr AG, Udey MC. Expression of gp40, the murine homologue of human epithelial cell adhesion molecule (Ep-CAM), by murine dendritic cells. Eur J Immunol. 1996; 26(1):110-114. (Clone-specific: Immunohistochemistry). View Reference
      5. Farr A, Nelson A, Truex J, Hosier S. Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium. J Histochem Cytochem. 1991; 39(5):345-353. (Immunogen: Electron microscopy, Immunohistochemistry, Immunoprecipitation). View Reference
      6. Litvinov SV, Balzar M, Winter MJ. Epithelial cell adhesion molecule (Ep-CAM) modulates cell-cell interactions mediated by classic cadherins. J Biol Chem. 1997; 139(5):1337-1348. (Biology). View Reference
      7. Nelson AJ, Dunn RJ, Peach R, Aruffo A, Farr AG. The murine homolog of human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells. Eur J Immunol. 1996; 26(2):401-408. (Clone-specific: Electron microscopy, Immunohistochemistry, Immunoprecipitation). View Reference
      8. Taguchi N, Hashimoto Y, Naiki M. Abnormal thymic expression of epithelial cell adhesion molecule (EP-CAM) in New Zealand Black (NZB) mice. J Autoimmun. 1999; 13(4):393-400. (Clone-specific: Electron microscopy). View Reference
      9. Zutter MM. Gastrointestinal carcinoma antigen GA733: target for immunodestruction and potential modifier of invasiveness and chemoresponsiveness. J Natl Cancer Inst. 1998; 90(9):642-644. (Biology). View Reference
      View All (9) View Less
      563216 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.