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BD Horizon™ BV510 Mouse Anti-NHP CD45
Clone D058-1283 (RUO)
Flow cytometric analysis of CD45 expression on Rhesus macaque peripheral blood leukocytes. Rhesus macaque whole blood was stained with BD Horizon™ BV510 Mouse Anti-NHP CD45 antibody (Cat. No. 563530; Right Panel) or with a BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; Left Panel). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two parameter flow cytometric dot plots showing the correlated expression of CD45 (or Ig Isotype control staining) versus side-scattered light signals were derived from events with the forward light scatter signals of viable leukocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of CD45 expression on Rhesus macaque peripheral blood leukocytes. Rhesus macaque whole blood was stained with BD Horizon™ BV510 Mouse Anti-NHP CD45 antibody (Cat. No. 563530; Right Panel) or with a BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; Left Panel). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two parameter flow cytometric dot plots showing the correlated expression of CD45 (or Ig Isotype control staining) versus side-scattered light signals were derived from events with the forward light scatter signals of viable leukocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of CD45 expression on Rhesus macaque peripheral blood leukocytes. Rhesus macaque whole blood was stained with BD Horizon™ BV510 Mouse Anti-NHP CD45 antibody (Cat. No. 563530; Right Panel) or with a BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; Left Panel). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two parameter flow cytometric dot plots showing the correlated expression of CD45 (or Ig Isotype control staining) versus side-scattered light signals were derived from events with the forward light scatter signals of viable leukocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of CD45 expression on Rhesus macaque peripheral blood leukocytes. Rhesus macaque whole blood was stained with BD Horizon™ BV510 Mouse Anti-NHP CD45 antibody (Cat. No. 563530; Right Panel) or with a BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; Left Panel). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two parameter flow cytometric dot plots showing the correlated expression of CD45 (or Ig Isotype control staining) versus side-scattered light signals were derived from events with the forward light scatter signals of viable leukocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Horizon™
Pan Leukocyte, NHP-specific; PTPRC; LCA; L-CA; Leukocyte Common Ag
Rhesus, Cynomolgus, Baboon (QC Testing)
Mouse IgG1, κ
Rhesus peripheral whole blood
Flow cytometry (Routinely Tested)
5 µl
AB_2738262
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- BD Horizon Brilliant Violet 510 is covered by one or more of the following US patents: 8,575,303; 8,354,239.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
BV510 Mouse IgG1, k Isotype Control RUO
Size
50 µg
Cat No.
562946
563530 Rev. 2
Antibody Details
D058-1283
D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes. It was developed using Rhesus peripheral whole blood as the immunogen. It does not cross-react with human leucocytes. This antibody reacts with baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern to CD45 binding to leukocyte common antigen (LCA) on human cells. Immunophenotypic analysis shows that D058-1283 binds to lymphocytes, monocytes and granulocytes of non-human primate blood samples. This antibody is able to block the binding of monoclonal antibody TÜ116; a reported anti-human CD45 antibody that cross-reacts with nonhuman primate leucocytes. In Western blot analysis, the D058-1283 antibody identifies a 180-200 kDa band.
563530 Rev. 2
Format Details
BV510
The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV510
327 nm, 405 nm
512 nm
563530 Rev.2
Citations & References
View product citations for antibody "563530" on CiteAb
Development References (3)
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Drouet M, Mayol JF, Norol F, et al. Lack of evidence of sustained hematopoietic reconstitution after transplantation of unmanipulated adult liver stem cells in monkeys. Haematologica. 2007; 92(2):248-251. (Clone-specific: Flow cytometry). View Reference
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Reeves RK, Evans TI, Gillis J, et al. Quantification of mucosal mononuclear cells in tissues with a fluorescent bead-based polychromatic flow cytometry assay. J Immunol Methods. 2011; 367(1-2):95-98. (Clone-specific: Flow cytometry). View Reference
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Reimann KA, Waite BC, Lee-Parritz DE, et al. Use of human leukocyte-specific monoclonal antibodies for clinically immunophenotyping lymphocytes of rhesus monkeys. Cytometry. 1994; 17(1):102-108. (Biology). View Reference
563530 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.