The RI7217 monoclonal antibody specifically recognizes the mouse Transferrin Receptor (TfR, Trfr, or TR), which is also known as Transferrin Receptor protein 1 (TfR1), CD71, and Mammary tumor virus receptor 1 (Mtvr-1 or Mtvr1). CD71 is a ~95-kDa single-pass type II transmembrane glycoprotein that is encoded by Tfrc and expressed on the cell surface as a disulfide-linked homodimer. The CD71 monomer is comprised of an extracellular C-terminal domain with a transferrin binding site followed by a transmembrane region and an N-terminal cytoplasmic tail that is involved in mediating the endocytosis and recycling of the receptor. This receptor mediates cellular iron uptake via endocytosis of an iron-transferrin complex followed by the recycling of transferrin and the receptor to the cell surface. CD71 is lowly expressed on most nonactivated cells including resting B and T lymphocytes. CD71 expression is upregulated on activated and proliferating cells such as mitogen- or antigen-stimulated B cells and T cells as well as some tumor cells. CD71 is variably expressed on erythroid precursors from pronormoblasts (proerythroblasts) to reticulocytes and is absent on mature red blood cells. CD71 is expressed on erythroid precursors that utilize iron for heme synthesis. CD71 can also serve as an entry receptor for several viral infections including those caused by Mouse mammary tumor virus (MMTV). The RI7217 antibody can reportedly be used to inhibit cellular proliferation.
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max near 407 nm and Em Max near 421 nm, BD Horizon BV421 can be excited by the violet laser (405 nm) and detected with a 450/50 nm filter. BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon V450 cannot be used simultaneously.