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BV421 Rat Anti-Mouse CD71
BV421 Rat Anti-Mouse CD71
Two-color flow cytometric analysis of CD71 expression on developing mouse erythroid cells. BALB/c mouse bone-marrow cells were simultaneously stained with PE Rat Anti-Mouse TER-119/Erythroid Cells (Cat. No. 553673) and with either BD Horizon™ BV421 Rat IgG1, κ Isotype Control (Cat. No. 562868, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD71 (Cat. No. 562716, Right Panel). The two-color flow cytometric dot plots showing the correlated expression of CD71 (or Ig Isotype control staining) versus TER-119 were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Two-color flow cytometric analysis of CD71 expression on developing mouse erythroid cells. BALB/c mouse bone-marrow cells were simultaneously stained with PE Rat Anti-Mouse TER-119/Erythroid Cells (Cat. No. 553673) and with either BD Horizon™ BV421 Rat IgG1, κ Isotype Control (Cat. No. 562868, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD71 (Cat. No. 562716, Right Panel). The two-color flow cytometric dot plots showing the correlated expression of CD71 (or Ig Isotype control staining) versus TER-119 were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
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BD Horizon™
Transferrin Receptor; TR; TfR; TfR1; Tfrc; Trfr; Mtvr-1
Mouse (QC Testing)
Rat WF, also known as Wistar Furth IgG1, κ
Mouse cell line
Flow cytometry (Routinely Tested)
0.2 mg/ml
22042
AB_2737742
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  8. Brilliant Violet™ 421 is a trademark of Sirigen.
562716 Rev. 1
Antibody Details
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C2

The C2 monoclonal antibody specifically binds to CD71, the transferrin receptor. CD71 is a disulfide-linked homodimer of 95-kDa subunits. CD71 mediates one of the cellular mechanisms for iron uptake, and its expression is regulated according to the cell's iron requirements. It is expressed at high levels on developing erythroid cells, and it is upregulated after mitogenic activation of B or T lymphocytes. The C2 monoclonal antibody selectivity inhibits some types of T- and B-cell activation by down-regulation of transferrin receptor expression, but it does not block binding of transferrin.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates..

562716 Rev. 1
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
562716 Rev.1
Citations & References
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Development References (5)

  1. Fujimoto T. GPI-anchored proteins, glycosphingolipids, and sphingomyelin are sequestered to caveolae only after crosslinking. J Histochem Cytochem. 1996; 44(8):929-941. (Clone-specific: Immunofluorescence). View Reference
  2. Kemp JD, Thorson JA, Gomez F, Smith KM, Cowdery JS, Ballas ZK. Inhibition of lymphocyte activation with anti-transferrin receptor Mabs: a comparison of three reagents and further studies of their range of effects and mechanism of action. Cell Immunol. 1989; 122(1):218-230. (Clone-specific: Activation, Inhibition). View Reference
  3. Kemp JD, Thorson JA, McAlmont TH, Horowitz M, Cowdery JS, Ballas ZK. Role of the transferrin receptor in lymphocyte growth: a rat IgG monoclonal antibody against the murine transferrin receptor produces highly selective inhibition of T and B cell activation protocols. J Immunol. 1987; 138(8):2422-2426. (Immunogen: Activation, Immunoprecipitation, Inhibition). View Reference
  4. Lok CN, Loh TT. Regulation of transferrin function and expression: review and update. Biol Signals Recept. 1998; 7(3):157-178. (Biology). View Reference
  5. Thorson JA, Smith KM, Gomez F, Naumann PW, Kemp JD. Role of iron in T cell activation: TH1 clones differ from TH2 clones in their sensitivity to inhibition of DNA synthesis caused by IgG Mabs against the transferrin receptor and the iron chelator deferoxamine. Cell Immunol. 1991; 134(1):126-137. (Clone-specific: Activation, Inhibition). View Reference
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562716 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.