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      BV421 Rat Anti-Mouse CD70
      BV421 Rat Anti-Mouse CD70
      Flow cytometric analysis of CD70 expression on activated mouse splenocytes. Splenic leucocytes from a BALB/c mouse were stimulated with lipopolysaccharide (LPS; 1 μg/ml; Sigma Cat. No. L-8272) for 3 days. The activated cells were stained with BD Horizon™ BV421  Rat IgG2b, κ Isotype Control (Cat. No. 562603; dashed line histogram) or BD Horizon™ BV421 Rat Anti-Mouse CD70 antibody (Cat. No. 562908; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      BV421 Rat Anti-Mouse CD70
      Flow cytometric analysis of CD70 expression on activated mouse splenocytes. Splenic leucocytes from a BALB/c mouse were stimulated with lipopolysaccharide (LPS; 1 μg/ml; Sigma Cat. No. L-8272) for 3 days. The activated cells were stained with BD Horizon™ BV421  Rat IgG2b, κ Isotype Control (Cat. No. 562603; dashed line histogram) or BD Horizon™ BV421 Rat Anti-Mouse CD70 antibody (Cat. No. 562908; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      Product Details
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      BD Horizon™
      CD27 ligand; CD27l, CD27LG; Tnfsf7; Ki-24 Antigen
      Mouse (QC Testing)
      Rat F344, also known as Fischer, CDF IgG2b, κ
      BALB/c mouse B lymphoma A20.2J
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_2737883
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
      8. Brilliant Violet™ 421 is a trademark of Sirigen.
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      562908 Rev. 1
      Antibody Details
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      FR70

      The FR70 monoclonal antibody specifically binds to mouse CD70, a 30-33-kDa type-II transmembrane glycoprotein belonging to the TNF/NGF superfamily. CD70 is the ligand for CD27 and is expressed primarily on activated B cells. CD70 expression can be induced by activation of splenocytes with lipopolysaccharide and stimulatory antibodies specific for IgM, CD40 or both. mRNA coding for CD70 is transiently expressed after Concanavalin A stimulation of splenocytes or thymocytes. CD70 has been observed to be expressed on dendritic cells of Leishmania major-infected mice. The CD27-CD70 interaction delivers costimulatory signals to T cells and NK cells. It has been reported that FR70 antibody blocks binding of mCD27-Ig fusion protein to mouse Cd70-transfected cells and inhibits some in vitro T-cell- and NK-cell-dependent responses.

      The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

      562908 Rev. 1
      Format Details
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      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      562908 Rev.1
      Citations & References
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      View product citations for antibody "562908" on CiteAb

      Development References (5)

      1. Akiba H, Miyahira Y, Atsuta M. Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis. J Exp Med. 2000; 191(2):375-382. (Biology). View Reference
      2. Hartwig UF, Karlsson L, Peterson PA, Webb SR. CD40 and IL-4 regulate murine CD27L expression. J Immunol. 1997; 159(12):6000-6008. (Biology). View Reference
      3. Oshima H, Nakano H, Nohara C. Characterization of murine CD70 by molecular cloning and mAb. Int Immunol. 1998; 10(4):517-526. (Immunogen). View Reference
      4. Takeda K, Oshima H, Hayakawa Y, et al. CD27-mediated activation of murine NK cells. J Immunol. 2000; 164(4):1741-1745. (Biology). View Reference
      5. Tesselaar K, Gravestein LA, van Schijndel GM, Borst J, van Lier RA. Characterization of murine CD70, the ligand of the TNF receptor family member CD27. J Immunol. 1997; 159(10):4959-4965. (Biology). View Reference
      View All (5) View Less
      562908 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.