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      BV421 Rat Anti-Mouse CD38
      BV421 Rat Anti-Mouse CD38
      Multicolor flow cytometric analysis of CD38 expression on C57BL/6 mouse splenocytes.  Splenic leucocytes were stained simultaneously with PE Hamster Anti-Mouse CD3 antibody (Cat. No. 553063/553064/561824) and with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD38 antibody (Cat. No. 562768; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD38 (or Ig Isotype control staining) versus CD3 for gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      BV421 Rat Anti-Mouse CD38
      Multicolor flow cytometric analysis of CD38 expression on C57BL/6 mouse splenocytes.  Splenic leucocytes were stained simultaneously with PE Hamster Anti-Mouse CD3 antibody (Cat. No. 553063/553064/561824) and with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD38 antibody (Cat. No. 562768; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD38 (or Ig Isotype control staining) versus CD3 for gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      Product Details
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      BD Horizon™
      ADP-ribosyl cyclase 1; Cyclic ADP-ribose hydrolase 1; I-19; NIM-R5
      Mouse (QC Testing)
      Rat IgG2a, κ
      Mouse Bone Marrow Pre-B cells
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      12494
      AB_2737781
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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      Recommended Assay Procedures

      For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
      8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      562768 Rev. 3
      Antibody Details
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      90/CD38

      The 90 monoclonal antibody specifically binds to CD38, a 42 kDa transmembrane glycoprotein on immature and mature, resting and activated, B lymphocytes. In contrast to humans, CD38 expression is down-regulated on mouse germinal center B cells and plasma cells. CD38 is also expressed on a subpopulation of thymic and peripheral T cells, NK cells, and splenic macrophages. Furthermore, CD38 has been detected on bone marrow-derived hematopoietic stem cells. The CD38 molecule is reported to exhibit both cyclase and hydrolase activities and plays a role in lymphocyte activation. CD31, both human and mouse, is reported to be a ligand for human CD38.

      562768 Rev. 3
      Format Details
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      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      562768 Rev.3
      Citations & References
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      Development References (11)

      1. BD Biosciences Pharmingen. Unpublished results. .
      2. Bean AG, Godfrey DI, Ferlin WG, et al. CD38 expression on mouse T cells: CD38 defines functionally distinct subsets of alpha beta TCR+CD4-CD8- thymocytes. Int Immunol. 1995; 7(2):213-221. (Biology). View Reference
      3. Cockayne DA, Muchamuel T, Grimaldi JC, et al. Mice deficient for the ecto-nicotinamide adenine dinucleotide glycohydrolase CD38 exhibit altered humoral immune responses. Blood. 1998; 92(4):1324-1333. (Biology). View Reference
      4. Deaglio S, Morra M, Mallone R, et al. Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member. J Immunol. 1998; 160(1):395-402. (Biology). View Reference
      5. Erickson LD, Vogel LA, Cascalho M, et al. B cell immunopoiesis: visualizing the impact of CD40 engagement on the course of T cell-independent immune responses in an Ig transgenic system. Eur J Immunol. 2000; 30(11):3121-3131. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
      6. Horenstein AL, Stockinger H, Imhof BA, Malavasi F. CD38 binding to human myeloid cells is mediated by mouse and human CD31. Biochem J. 1998; 330(3):1129-1135. (Biology). View Reference
      7. Howard M, Grimaldi JC, Bazan JF, et al. Formation and hydrolysis of cyclic ADP-ribose catalyzed by lymphocyte antigen CD38. Science. 1993; 262(5136):1056-1059. (Biology). View Reference
      8. Lund F, Solvason N, Grimaldi JC, Parkhouse RM, Howard M. Murine CD38: an immunoregulatory ectoenzyme. Immunol Today. 1995; 16(10):469-473. (Biology). View Reference
      9. Oliver AM, Martin F, Kearney JF. Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells. J Immunol. 1997; 158(3):1108-1115. (Immunogen: ELISA, Flow cytometry, Fluorescence activated cell sorting, IC/FCM Block, Immunofluorescence, Immunoprecipitation). View Reference
      10. Oliver AM. Personal Communication. .
      11. Randall TD, Lund FE, Howard MC, Weissman IL. Expression of murine CD38 defines a population of long-term reconstituting hematopoietic stem cells. Blood. 1996; 87(10):4057-4067. (Biology). View Reference
      View All (11) View Less
      562768 Rev. 3

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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