BV421 Rat Anti-Mouse CD161f

This is a suggested alternate SKU for 566304. Contact us for additional support.

BD OptiBuild™ BV421 Rat Anti-Mouse CD161f

Name: BV421 Rat Anti-Mouse CD161f
Brand: BD OptiBuild™
SKU: 752796

Clone C8

(RUO)

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD OptiBuild™

Alternative Name: Klrb1f; Nkrp1-f; Nkrp1f; NKR-P1F

Reactivity: Mouse (Tested in Development)

Isotype: Rat PVG, also known as HO, Black Hooded IgG1, κ

Immunogen: Mouse NKRP1F Transfected Cell Line

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Researchers should determine the optimal concentration of this reagent for their individual applications.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Pacific Blue™ is a trademark of Life Technologies Corporation.
BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.

Companion Products

Brilliant Stain Buffer RUO

Size 1000 Tests Cat No. 566349

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Brilliant Stain Buffer RUO

Size 100 Tests Cat No. 563794

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Brilliant Stain Buffer Plus RUO

Size 1000 Tests Cat No. 566385

Lysing Buffer RUO

Size 100 mL Cat No. 555899

View Details

752796 Rev. 1

Antibody Details

The C8 monoclonal antibody specifically recognizes C57BL/6 mouse CD161 antigen-like family member F (CD161f ). CD161f is also known as Natural killer cell surface protein NKR-P1F (Nkrp1f), and is encoded by Klrb1f (Killer cell lectin-like receptor subfamily B member 1F). CD161f is expressed on natural killer (NK) cells, and some dendritic cells and activated lymphocytes. CD161f binds to C-type lectin related protein c, d, and g (Clr-c, d, g) which are expressed on several cell types including dendritic cells, B cells, and activated T cells. CD161f-mediated signaling can reportedly costimulate T cell proliferation and IL-2 production, and may play a role in dendritic cell maturation.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max near 407 nm and Em Max near 421 nm, BD Horizon BV421 can be excited by the violet laser (405 nm) and detected with a 450/50 nm filter. BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon V450 cannot be used simultaneously.

752796 Rev. 1

Format Details

BV421

The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: BV421

Excitation Source: Violet 405 nm

Excitation Max: 407 nm

Emission Max: 423 nm

752796 Rev.1

Citations & References

Development References (9)

Aust JG1, Gays F, Mickiewicz KM, Buchanan E, Brooks CG.. The expression and function of the NKRP1 receptor family in C57BL/6 mice.. J Immunol. 2009; 183(1):106-116. (Immunogen: Flow cytometry). View Reference
Carlyle JR, Mesci A, Ljutic B, et al. Molecular and genetic basis for strain-dependent NK1.1 alloreactivity of mouse NK cells.. J Immunol. 2006; 176(12):7511-24. (Biology). View Reference
Chai L, Wu S, Liu G, Wang Z, Tian W, Ma Y. OCILRP2 signaling synergizes with LPS to induce the maturation and differentiation of murine dendritic cells.. Biochem Biophys Res Commun. 2014; 446(4):836-42. (Biology). View Reference
Iizuka K, Naidenko OV, Plougastel BF, Fremont DH, Yokoyama WM. Genetically linked C-type lectin-related ligands for the NKRP1 family of natural killer cell receptors.. Nat Immunol. 2003; 4(8):801-7. (Biology). View Reference
Kirkham CL, Carlyle JR. Complexity and Diversity of the NKR-P1:Clr (Klrb1:Clec2) Recognition Systems. Front Biosci. 2014; 5(5):1-16. (Biology). View Reference
Plougastel B, Matsumoto K, Dubbelde C, Yokoyama WM. Analysis of a 1-Mb BAC contig overlapping the mouse Nkrp1 cluster of genes: cloning of three new Nkrp1 members, Nkrp1d, Nkrp1e, and Nkrp1f.. Immunogenetics. 2001; 53(7):592-8. (Biology). View Reference
Rozbeský D, Ivanova L, Hernychová L, Grobárová V, Novák P, Černý J. Nkrp1 family, from lectins to protein interacting molecules.. Molecules. 2015; 20(2):3463-78. (Clone-specific). View Reference
Tian W, Feng B, Liou HC. Silencing OCILRP2 leads to intrinsic defects in T cells in response to antigenic stimulation.. Cell Immunol. 2005; 235(1):72-84. (Biology). View Reference
Tian W, Nunez R, Cheng S, et al. C-type lectin OCILRP2/Clr-g and its ligand NKRP1f costimulate T cell proliferation and IL-2 production.. Cell Immunol. 2005; 234(1):39-53. (Biology). View Reference

View All (9)

752796 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.