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BD Horizon™ BV421 Mouse Anti-Rat CD3
Clone 1F4 (RUO)
Two-color flow cytometric analysis of CD3 expression on rat splenocytes. Rat splenic leucocytes were stained with PE Mouse Anti-Rat CD5 antibody (Cat. No. 554851) and either BD Horizon™ BV421 Mouse IgM, κ Isotype Control (Cat. No. 562704; Left Panel) or BD Horizon™ BV421 Mouse Anti-Rat CD3 antibody (Cat. No. 563948; Right Panel). The two-color flow cytometric dot plot shows the correlated expression patterns of CD3 (or Ig Isotype control staining) versus CD5 for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes.
Two-color flow cytometric analysis of CD3 expression on rat splenocytes. Rat splenic leucocytes were stained with PE Mouse Anti-Rat CD5 antibody (Cat. No. 554851) and either BD Horizon™ BV421 Mouse IgM, κ Isotype Control (Cat. No. 562704; Left Panel) or BD Horizon™ BV421 Mouse Anti-Rat CD3 antibody (Cat. No. 563948; Right Panel). The two-color flow cytometric dot plot shows the correlated expression patterns of CD3 (or Ig Isotype control staining) versus CD5 for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes.
Two-color flow cytometric analysis of CD3 expression on rat splenocytes. Rat splenic leucocytes were stained with PE Mouse Anti-Rat CD5 antibody (Cat. No. 554851) and either BD Horizon™ BV421 Mouse IgM, κ Isotype Control (Cat. No. 562704; Left Panel) or BD Horizon™ BV421 Mouse Anti-Rat CD3 antibody (Cat. No. 563948; Right Panel). The two-color flow cytometric dot plot shows the correlated expression patterns of CD3 (or Ig Isotype control staining) versus CD5 for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes.
Two-color flow cytometric analysis of CD3 expression on rat splenocytes. Rat splenic leucocytes were stained with PE Mouse Anti-Rat CD5 antibody (Cat. No. 554851) and either BD Horizon™ BV421 Mouse IgM, κ Isotype Control (Cat. No. 562704; Left Panel) or BD Horizon™ BV421 Mouse Anti-Rat CD3 antibody (Cat. No. 563948; Right Panel). The two-color flow cytometric dot plot shows the correlated expression patterns of CD3 (or Ig Isotype control staining) versus CD5 for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes.
Product Details
BD Horizon™
Rat (QC Testing)
Mouse BALB/c IgM, κ
F344 rat splenic T cells stimulated with PMA plus A23187
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738503
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- Brilliant Violet™ 421 is a trademark of Sirigen.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
BV421 Mouse IgM, κ Isotype Control RUO
Size
50 µg
Cat No.
562704
563948 Rev. 1
Antibody Details
1F4
The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation. Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat. No. 559974/554830).
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.
563948 Rev. 1
Format Details
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
563948 Rev.1
Citations & References
View product citations for antibody "563948" on CiteAb
Development References (3)
-
Elbe A, Kilgus O, Hünig T, and Stingl G. T-cell receptor diversity in dendritic epidermal T cells in the rat. J Invest Dermatol. 1993; 102:74-79. (Biology). View Reference
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Nicolls MR, Aversa GG, Pearce NW, et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.. Transplantation. 1993; 55(3):459-68. (Biology). View Reference
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Tanaka T, Masuko T, Yagita H, Tamura T, Hashimoto Y. Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.. J Immunol. 1989; 142(8):2791-2795. (Immunogen: Flow cytometry, Functional assay, Immunohistochemistry, Stimulation). View Reference
563948 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.