The NLDC-145 monoclonal antibody specifically recognizes CD205 which is also known as Dendritic and thymic Epithelial Cells, 205 kDa (DEC-205) or Lymphocyte antigen 75 (Ly-75). CD205 (DEC-205) is a ~205 kDa type 1 transmembrane glycoprotein that is encoded by Ly75 which belongs to the mannose receptor family within the C-type lectin superfamily. The extracellular region contains an N-terminal cysteine-rich domain followed by a fibronectin type II domain, 10 C-type lectin-like domains, a transmembrane region and cytoplasmic tail with an internalization sequence. CD205 (DEC-205) is highly expressed on both CD8+ and CD103+ dendritic cell subsets and Langerhans cells. It is variably expressed by some other dendritic cell types, Gr1+ bone marrow cells, B cells, T cells, thioglycolate-elicited macrophages, granulocytes, mucosal and thymic cortical epithelial cells, and brain endothelial cells. CD205 (DEC-205) serves as a pattern recognition receptor that functions as a recycling endocytic receptor. It participates in the cellular processing, presentation, and cross-presentation of foreign or self-antigens to initiate immunity or tolerance, respectively. CD205 (DEC-205) can reportedly serve as a promising target for immunotherapy including DC-targeted vaccination using anti-CD205 (DEC-205) monoclonal antibody conjugated with antigen for T cell activation.
The antibody was conjugated to BD Horizon BUV661 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 661-nm. BD Horizon Brilliant BUV661 can be excited by the ultraviolet laser (355 nm) and detected with a 670/25 filter and a 630 nm LP. Due to cross laser excitation of this dye, there may be significant spillover into channels detecting APC-like emissions (eg, 670/25-nm filter).