The M89D3 monoclonal antibody specifically recognizes CD31, which is also known as platelet endothelial cell adhesion molecule -1 (PECAM-1), a single chain, type I transmembrane protein of approximately 130-140 kDa. CD31 is a member of the immunoglobulin supergene family. Its extracellular region is composed of 574 amino acids and contains six C2-type Ig domains, and the intracellular region is composed of 118 residues. CD31 (PECAM-1) is expressed on endothelial cells, platelets, monocytes, neutrophils and NK cells. It has also been observed on subsets of T cells, but not on circulating B cells. It has been implicated in a number of cellular phenomena, including vascular wound healing, angiogenesis, and transendothelial migration of leukocytes and platelet aggregation in inflammatory responses.
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.