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![Biotin Mouse Anti-Pig CD16](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/551xxx/5513xx/551395_base/551395Image1.png)
![Biotin Mouse Anti-Pig CD16](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/551xxx/5513xx/551395_base/551395Image1.png)
![](/content/dam/bdb/products/vialimages/packaging-images/black-vial-images/Biotin.jpg)
![Biotin Mouse Anti-Pig CD16](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/551xxx/5513xx/551395_base/551395Image1.png)
Two-color analysis of CD16 expression on pig peripheral blood lymphocytes. Pig whole blood was stained with PE-conjugated anti- pig CD4a mAb 74-12-4 (Cat. no. 559586, both panels) and biotinylated mAb FcG7 (Right panel), followed by Streptavidin- FITC (Cat. No. 554060, both panels). Erythrocytes were lysed (PharmLyse™ Lysing Buffer, Cat. no. 555899), non-viable leukocytes were excluded by staining with 7-AAD (Via- Probe™, Cat. No. 555816/555815), and lymphocytes were gated according to their light-scatter profiles. Flow cytometry was performed on a FACSCaliburª (BDIS, San Jose, CA).
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![Biotin Mouse Anti-Pig CD16](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/551xxx/5513xx/551395_base/551395Image1.png)
BD Pharmingen™ Biotin Mouse Anti-Pig CD16
![](/content/dam/bdb/products/vialimages/packaging-images/black-vial-images/Biotin.jpg)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The FcG7 antibody reacts with CD16, the transmembrane α chain of Fcγ Receptor III (FcγRIIIAα). In the pig, FcγRIIIAα is expressed on NK cells, monocytes, granulocytes, and pulmonary alveolar macrophages. The FcG7 antibody induces redirected cytotoxic activity of NK cells and granulocytes against tumor cells.
Development References (5)
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Aller SC, Cho D, Kim YB. Characterization of the cytolytic trigger molecules G7/PNK-E as a molecular complex on the surface of porcine phagocytes. Cell Immunol. 1995; 161(2):270-278. (Biology). View Reference
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Dato ME, Wierda WG, Kim YB. A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes. Cell Immunol. 1992; 140(2):468-477. (Immunogen: Cytotoxicity). View Reference
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Halloran PJ, Sweeney SE, Kim YB. Biochemical characterization of the porcine Fc gamma RIII alpha homologue G7. Cell Immunol. 1994; 158(2):400-413. (Biology). View Reference
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Halloran PJ, Sweeney SE, Strohmeier CM, Kim YB. Molecular cloning and identification of the porcine cytolytic trigger molecule G7 as a Fc gamma RIII alpha (CD16) homologue. J Immunol. 1994; 153(6):2631-2641. (Biology). View Reference
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Wierda WG, Johnson BD, Dato ME, Kim YB. Induction of porcine granulocyte-mediated tumor cytotoxicity by two distinct monoclonal antibodies against lytic trigger molecules (PNK-E/G7). J Immunol. 1993; 151(12):7117-7127. (Clone-specific: Cytotoxicity). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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