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Biotin Mouse Anti-Human CD62E
Product Details
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BD Pharmingen™
SELE; E-selectin; Selectin E; ELAM; ELAM1; ESEL; LECAM2
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
0.5 mg/ml
VI A090
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
555649 Rev. 7
Antibody Details
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The 68-5H11 monoclonal antibody specifically binds to CD62E. This adhesion molecule is a 97-115 kDa type I transmembrane glycoprotein that is encoded by the SELE gene. CD62E is also known as E-selectin, Endothelial-leukocyte adhesion molecule-1 (ELAM-1), and Leukocyte endothelial cell adhesion molecule 2 (LECAM-2). CD62E is minimally expressed by unstimulated endothelium. Activated endothelial cells upregulate surface CD62E expression in response to various activators including inflammatory cytokines such as Interleukin-1 and Tumor Necrosis Factor. CD62E plays a role in leucocyte extravasation by promoting leucocyte rolling on activated endothelial cells during inflammation. CD62E may also play a role in the metastasis of certain tumor cells. The adhesion between endothelial CD62E molecules and a carbohydrate ligand on neutrophils is inhibited by the mAb 68-5H11.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

555649 Rev. 7
Format Details
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Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
555649 Rev.7
Citations & References
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Development References (5)

  1. Bevilacqua MP, Stengelin S, Gimbrone MA Jr, Seed B. Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science. 1989; 243(4895):1160-1165. (Biology). View Reference
  2. Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-1139. (Biology). View Reference
  3. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
  4. Phillips ML, Nudelman E, Gaeta FC, et al. ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex. Science. 1990; 250(4984):1130-1132. (Biology). View Reference
  5. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
View All (5) View Less
555649 Rev. 7

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.