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APC Mouse Anti-Rat CD11b
APC Mouse Anti-Rat CD11b

Flow cytometric analysis of CD11b expression on rat bone marrow cells.  Lewis rat bone marrow cells were stained with either APC Mouse IgA, κ Isotype Control (Cat. No. 562140, Left Panel) or an APC Mouse Anti-Rat CD11b antibody (Cat.No. 562102, Right Panel). Two-parameter flow cytometric dot blots showing side-scattered light signals versus CD11b (or Ig Isotype Control staining) were derived from gated events with the forward light-scatter characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.  

Flow cytometric analysis of CD11b expression on rat bone marrow cells.  Lewis rat bone marrow cells were stained with either APC Mouse IgA, κ Isotype Control (Cat. No. 562140, Left Panel) or an APC Mouse Anti-Rat CD11b antibody (Cat.No. 562102, Right Panel). Two-parameter flow cytometric dot blots showing side-scattered light signals versus CD11b (or Ig Isotype Control staining) were derived from gated events with the forward light-scatter characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.  

Product Details
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BD Pharmingen™
Itgam; Integrin αM chain; Mac-1 α chain; Integrin beta 2 alpha subunit
Rat (QC Testing)
Mouse BALB/c IgA, κ
Rat neutrophils
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_10896148
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
562102 Rev. 1
Antibody Details
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WT.5

The WT.5 monoclonal antibody specifically binds to CD11b, the α subunit of Mac-1 found on neutrophils and myeloid cells, but not on lymphocytes. Mac-1 expression is rapidly upregulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. Mac-1 mediates adhesion to ICAM-1 (CD54) and C3bi. Neutrophil binding to ICAM-1 is blocked by mAb WT.5.

562102 Rev. 1
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
562102 Rev.1
Citations & References
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Development References (1)

  1. Tamatani T, Kitamura F, Kuida K, et al. Characterization of rat LECAM-1 (L-selectin) by the use of monoclonal antibodies and evidence for the presence of soluble LECAM-1 in rat sera. Eur J Immunol. 1993; 23(9):2181-2188. (Immunogen). View Reference
562102 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.