Flow cytometric analysis of CPT1A expression in MCF-7 cells. Cells from the Human MCF-7 (Breast adenocarcinoma, ATCC® HTB-22™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with Alexa Fluor™ 647 Mouse IgG2b, κ Isotype Control (Cat. No. 565378; dashed line histogram) or Alexa Fluor™ 647 Rabbit Anti-Human CPT1A antibody (Cat. No. 569343; solid line histogram) at 0.25 µg/test. The fluorescence histogram showing the expression of CPT1A (or Ig Isotype control staining) was derived from gated events with the forward and side-light scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of CPT1A expression in MCF-7 cells. Cells from the Human MCF-7 (Breast adenocarcinoma, ATCC® HTB-22™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with Alexa Fluor™ 647 Mouse IgG2b, κ Isotype Control (Cat. No. 565378; dashed line histogram) or Alexa Fluor™ 647 Rabbit Anti-Human CPT1A antibody (Cat. No. 569343; solid line histogram) at 0.25 µg/test. The fluorescence histogram showing the expression of CPT1A (or Ig Isotype control staining) was derived from gated events with the forward and side-light scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
BD Pharmingen™
CPT I; CPT1; CPT1-L; CPT1A; CPTI-L; L-CPT1
Human (QC Testing)
Mouse IgG2b, κ
Human CPT1A (Amino Acids 489-773)
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
1374
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
- This product is, in part, provided under an intellectual property license from Abcam. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product for buyer’s internal research use only (whether the buyer is an academic or for-profit entity) and buyer shall not be permitted to reverse engineer or otherwise perform any compositional, structural, functional or other analyses directed to learning the methodology, formulae, sequences, process, make-up or production of this product (or any portion thereof). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. If you, as the buyer, do not agree to these conditions, please leave the product unopened and return the contents of this package to BD Biosciences, 10975 Torreyana Road, San Diego, CA 92121. For information on purchasing a license to this product for purposes other than research, contact Abcam at partnerships@abcam.com.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control RUO
Size
50 µg
Cat No.
565378
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
Perm Buffer III RUO
Size
125 mL
Cat No.
558050
569343 Rev. 1
Antibody Details
8F6AE9
The 8F6AE9 monoclonal antibody specifically recognizes Carnitine palmitoyltransferase 1A (CPT1A), also known as, Carnitine O-palmitoyltransferase 1, liver isoform. CPT1A is a ~ 88 kDa enzyme encoded by CPT1A which belongs to the carnitine/choline acetyltransferase family. CPT1A is located in the mitochondrial outer membrane but facilitates carnitine-dependent transport of fatty acids across the inner mitochondrial membrane and plays a key role in triglyceride metabolism. CPT1A is the primary isoform and is found in the liver, spleen, kidneys, lungs, intestines, pancreas, brain and ovaries. Overexpression of CPT1A has been reported in tumor cell lines and its deficiency is associated with many metabolic disorders. Recently, CPTIA has been suggested as a novel prognostic indicator and potential target for anti-cancer therapy.
569343 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
569343 Rev.1
Citations & References
View product citations for antibody "569343" on CiteAb
Development References (8)
-
Carrier B, Wen S, Zigouras S, et al. Alpha-Lipoic Acid Reduces LDL-Particle Number and PCSK9 Concentrations in High-Fat Fed Obese Zucker Rats.. PLoS ONE. 2014; 9(3):e90863. (Clone-specific: Western blot). View Reference
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Ahl PJ, Hopkins RA, Xiang WW, et al. Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations.. Communications Biology. 2020; 3(1):1-15. (Clone-specific: Flow cytometry). View Reference
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Byersdorfer CA, Tkachev V, Opipari AW, et al. Effector T cells require fatty acid metabolism during murine graft-versus-host disease.. Blood. 2013; 122(18):3230-7. (Clone-specific: Flow cytometry). View Reference
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Gamradt S, Hasselmann H, Taenzer A, et al. Reduced mitochondrial respiration in T cells of patients with major depressive disorder.. iScience. 2021; 24(11):103312. (Clone-specific: Flow cytometry). View Reference
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Schlaepfer IR, Joshi M. CPT1A-mediated Fat Oxidation, Mechanisms, and Therapeutic Potential.. Endocrinology. 2020; 161(2):bqz046. (Biology). View Reference
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Stefanovic-Racic M, Perdomo G, Mantell BS, Sipula IJ, Brown NF, O'Doherty RM. A moderate increase in carnitine palmitoyltransferase 1a activity is sufficient to substantially reduce hepatic triglyceride levels.. Am J Physiol Endocrinol Metab. 2008; 294(5):E969-77. (Biology). View Reference
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Wang M, Wang K, Liao X, et al. Carnitine Palmitoyltransferase System: A New Target for Anti-Inflammatory and Anticancer Therapy?. Front Pharmacol. 2021; 12:760581. (Biology). View Reference
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Xu Y, Wu G, Ma X, et al. Identification of CPT1A as a Prognostic Biomarker and Potential Therapeutic Target for Kidney Renal Clear Cell Carcinoma and Establishment of a Risk Signature of CPT1A-Related Genes.. Int J Genomics. 2020; 2020:9493256. (Biology). View Reference
569343 Rev. 1
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.