Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
India (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Alexa Fluor® 647 Mouse Anti-FoxP2
      Alexa Fluor® 647 Mouse Anti-FoxP2
      Flow cytometric analysis of FoxP2 expression in human RPMI-8226. Cells from the human RPMI-8226 (Plasmacytoma, ATCC CCL-155) cell line were fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) and stained with matching concentrations of either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557732; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-FoxP2 antibody (Cat. No. 564463; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact RPMI-8226 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometer System.
      Alexa Fluor® 647 Mouse Anti-FoxP2
      Image analysis of FoxP2 expression by Purkinje cells within the mouse cerebellum. Following antigen retrieval with BD Retrievagen A (Cat. No. 550524), sections prepared from formalin-fixed and paraffin-embedded mouse cerebellum were stained with matching concentrations of Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Upper Panel) or Alexa Fluor® 647 Mouse Anti-FoxP2 antibody (Lower Panel; Pseudocolored Red). The tissues were counterstained with Alexa Fluor® 488 Mouse Anti-β-Tubulin, Class III antibody (Cat. No. 560338; Pseudocolored Green) and Hoechst 33342 Solution (Cat. No. 561908; Pseudocolored Blue). The Purkinje cell nuclei stained positively for the expression of FoxP2.  Images were analyzed using a BD Pathway™ 435 High-Content Bioimager System and merged using BD Attovision™ Software.
      Alexa Fluor® 647 Mouse Anti-FoxP2 Alexa Fluor® 647 Mouse Anti-FoxP2
      Flow cytometric analysis of FoxP2 expression in human RPMI-8226. Cells from the human RPMI-8226 (Plasmacytoma, ATCC CCL-155) cell line were fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) and stained with matching concentrations of either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557732; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-FoxP2 antibody (Cat. No. 564463; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact RPMI-8226 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometer System.
      Image analysis of FoxP2 expression by Purkinje cells within the mouse cerebellum. Following antigen retrieval with BD Retrievagen A (Cat. No. 550524), sections prepared from formalin-fixed and paraffin-embedded mouse cerebellum were stained with matching concentrations of Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Upper Panel) or Alexa Fluor® 647 Mouse Anti-FoxP2 antibody (Lower Panel; Pseudocolored Red). The tissues were counterstained with Alexa Fluor® 488 Mouse Anti-β-Tubulin, Class III antibody (Cat. No. 560338; Pseudocolored Green) and Hoechst 33342 Solution (Cat. No. 561908; Pseudocolored Blue). The Purkinje cell nuclei stained positively for the expression of FoxP2.  Images were analyzed using a BD Pathway™ 435 High-Content Bioimager System and merged using BD Attovision™ Software.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      TNRC10; SPCH1; CAGH44
      Human (QC Testing), Mouse (Tested in Development)
      Mouse BALB/c IgG1, κ
      Human FoxP2 Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested), Bioimaging (Tested During Development)
      0.2 mg/ml
      AB_2738816
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      Show More blue down arrow Show Less blue up arrow
      564463 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      FOXP2-73A/8

      The FOXP2-73A/8 monoclonal antibody specifically binds to Forkhead box protein P2 (FoxP2). FoxP2 is also known as TNRC10 (Trinucleotide repeat-containing gene 10 protein), CAGH44 (CAG repeat protein 44), and SPCH1 (Speech and Language Disorder 1). FoxP2 is a member of the forkhead/winged-helix (FOX) family of transcription factors. Normal FOXP2 gene expression is associated with the proper development of cardiovascular, gastrointestinal, and neural tissues including the speech and language regions of the brain. FoxP2 protein has been detected in most normal tissues including epithelial and endothelial cells but not cells of hematopoietic origin. Although not normally detected in lymphocytes and plasma cells, FoxP2 protein was reportedly expressed in a small fraction of plasma cells from reactive human tonsils. Aberrant FOXP2 gene expression has been associated with abnormal plasma cell generation and may play a role in multiple myeloma and other hematological malignancies. The FOXP2-73A/8 antibody detects human and mouse FoxP2 and does not crossreact with other members of the FOXP family (FoxP1, FoxP3 or FoxP4).

      564463 Rev. 1
      Format Details
      Down Arrow Up Arrow
      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      564463 Rev.1
      Citations & References
      Down Arrow Up Arrow

      Development References (2)

      1. Campbell AJ, Lyne L, Brown PJ, et al. Aberrant expression of the neuronal transcription factor FOXP2 in neoplastic plasma cells. Br J Haematol. 2010; 149(2):221-230. (Immunogen: Immunohistochemistry, Western blot). View Reference
      2. Lai CS, Fisher SE, Hurst JA, Vargha-Khadem F, Monaco AP. A forkhead-domain gene is mutated in a severe speech and language disorder. Nature. 2001; 413(6855):519-523. (Biology). View Reference
      564463 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback