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NHP Compensation Set

BD Pharmingen™ NHP Compensation Set

Product Details
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BD Pharmingen™
Flow cytometry (Routinely Tested)


The Non-Human Primate (NHP) compensation set is intended to be used with the NHP T lymphocyte Cocktail (Cat. no. 558625) or the NHP T/B/NK Cell Cocktail (Cat. no. 558639) for instrument compensation.  Compensation is the process by which the effect of spectral overlap among flurochromes can be corrected minimizing fluorescence signal spillover in neighboring channels.  Traditionally, compensation values are entered into software programs allowing for the adjustment among the overlapping flurochrome emissions.  These values can be achieved using single color controls, and verified using fluorescence-minus-one controls, or ad-mixtures of single color controls.  The non-human primate (NHP) compensation set is made up of FITC Mouse anti-Human CD3 (clone SP34-2),  APC Mouse anti-Human CD8 (clone SK1) and PE Mouse anti-Human CD4 (clone L200). Use this set to properly adjust your FITC/PE/APC compensation matrix for the NHP T Lymphocyte or NHP T/B/NK cell cocktails.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Please refer to for technical protocols.
558640 Rev. 2
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Description Quantity/Size Part Number EntrezGene ID
PE Mouse anti-Human CD4 20 Tests (1 ea) 51-9004313 N/A
FITC Mouse anti-Human CD3 20 Tests (1 ea) 51-9004315 N/A
APC Mouse anti-Human CD8 20 Tests (1 ea) 51-9004954 N/A
558640 Rev. 2
Citations & References
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Development References (1)

  1. Shapiro HM. Practical flow cytometry, 3rd ed.. New York: Wiley-Liss; 1995:18-19.
558640 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.