Skip to main content Skip to navigation
APC Mouse Lineage Antibody Cocktail, with Isotype Control

APC Mouse Lineage Antibody Cocktail, with Isotype Control

(RUO)
APC Mouse Lineage Antibody Cocktail, with Isotype Control
Identification of CD34+ and CD34- subpopulations of hematopoietic progenitors. BALB/c bone marrow cells were treated with Mouse BD Fc Block™ Purified anti-CD16/CD32  (Cat. No. 553141/553142), stained with FITC Rat anti-Mouse CD34  (Cat. No. 553733) and either APC Mouse Lineage Isotype Control Cocktail (left panel) or APC Mouse Lineage Antibody Cocktail (right panel, Cat. No. 558074). Dead cells were excluded from analysis by staining with Propidium Iodide Staining Solution (Cat. No. 556463). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Identification of CD34+ and CD34- subpopulations of hematopoietic progenitors. BALB/c bone marrow cells were treated with Mouse BD Fc Block™ Purified anti-CD16/CD32  (Cat. No. 553141/553142), stained with FITC Rat anti-Mouse CD34  (Cat. No. 553733) and either APC Mouse Lineage Isotype Control Cocktail (left panel) or APC Mouse Lineage Antibody Cocktail (right panel, Cat. No. 558074). Dead cells were excluded from analysis by staining with Propidium Iodide Staining Solution (Cat. No. 556463). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Product Details
Down Arrow Up Arrow


BD Pharmingen™
Mouse (QC Testing)
Flow cytometry (Routinely Tested)
RUO
AB_1645213


Description

The APC Mouse Lineage Antibody Cocktail has been designed to react with cells from the major hematopoietic lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, NK cells, erythrocytes, and granulocytes. This pre-diluted Cocktail of five APC-conjugated antibodies is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include clone 145-2C11, which recognizes Mouse CD3e; M1/70, which  recognizes CD11b; RA3-6B2, which recognizes CD45R/B220; TER-119, which recognizes Ly-76, mouse erythroid cells; and RB6-8C5, which recognizes Ly-6G and Ly-6C. APC Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative-control immunoglobulin. Additional fluorochrome-labeled reagents may be combined with the APC Mouse Lineage Antibody Cocktail, and the APC Mouse Lineage Isotype Control Cocktail, to further characterize hematopoietic progenitor subpopulations.  

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Recommended Assay Procedures

20 µl of the antibody or isotype control cocktail is sufficient to stain each sample of 10^6 leukocytes for flow cytometric analysis. We recommend the use of Mouse BD Fc Block™ purified anti-mouse CD16/Cd32 (Cat. No. 553141/553142) for optimal staining. The APC Mouse Lineage Antibody Cocktail can be used to deplete cells bearing the hematopoietic lineage markers by flow cytometric or immunomagnetic (using BD™ IMag anti-Allophycocyanin (APC) Particles - DM, Cat. No. 557932) sorting. The APC fluorochrome is excited by laser lines from 595 to 647 nm, and its emission is collected in a detector for fluorescence wavelengths between 640 and 680 nm.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558074 Rev. 4
Components
Down Arrow Up Arrow
Description Quantity/Size Part Number EntrezGene ID
APC Mouse Lineage Antibody Cocktail 100 Tests (1 ea) 51-9003632 N/A
APC Mouse Lineage Isotype Control Cocktail 100 Tests (1 ea) 51-9003633 N/A
558074 Rev. 4
Citations & References
Down Arrow Up Arrow

Development References (6)

  1. Goodell MA, Rosenzweig M, Kim H, et al. Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species. Nat Med. 1997; 3(12):1337-1345. (Biology). View Reference
  2. Morrison SJ, Wandycz AM, Hemmati HD, Wright DE, Weissman IL. Identification of a lineage of multipotent hematopoietic progenitors. Development. 1997; 124(10):1929-1939. (Biology). View Reference
  3. Okada S, Nakauchi H, Nagayoshi K, Nishikawa S, Miura Y, Suda T. In vivo and in vitro stem cell function of c-kit- and Sca-1-positive murine hematopoietic cells. Blood. 1992; 80(12):3044-3050. (Biology). View Reference
  4. Osawa M, Tokumoto Y, Nakauchi H. Hematopoietic stem cells. In: Herzenberg LA, Weir DM, Blackwell C, ed. Weir's Handbook of Experimental Immunology, 5th Edition. Cambridge: Blackwell Science; 1996:66.1-66.5.
  5. Spangrude GJ, Heimfeld S, Weissman IL. Purification and characterization of mouse hematopoietic stem cells. Science. 1988; 241(4861):58-62. (Biology). View Reference
  6. Spangrude GJ, Scollay R. A simplified method for enrichment of mouse hematopoietic stem cells. Exp Hematol. 1990; 18(8):920-926. (Biology). View Reference
View All (6) View Less
558074 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.