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PerCP-Cy™5.5 Mouse Anti-Human CD79a
Product Details
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BD™
CD79a, immunoglobulin-associated alpha ; CD79A; Ig-alpha; IGA; Igα; MB-1
Human
Mouse BALB/c IgG1, κ
Human CD79a Peptide
Flow cytometry
3.0 μg/mL
20 μL
V cB017
973
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

341643 Rev. 1
Antibody Details
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HM47

The CD79a antibody, clone HM47, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells from BALB/c mice immunized with a synthetic peptide conjugated to thyroglobulin. The peptide has the sequence representing amino acids 202–216 inclusive as deduced from the human mb-1 cDNA sequence.

The CD79a antibody recognizes the cytoplasmic domain of a 47-kilodalton (kDa) membrane glycoprotein present on B lymphocytes.

341643 Rev. 1
Format Details
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PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
341643 Rev.1
Citations & References
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View product citations for antibody "341643" on CiteAb

Development References (7)

  1. Astsaturov IA, Matutes E, Morilla R, et al. Differential expression of B29 (CD79b) and mb-1 (CD79a) proteins in acute lymphoblastic leukaemia. Leukemia. 1996; 10(5):769-773. (Biology). View Reference
  2. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  3. Clinical and Laboratory Standards Institute. 2005. (Biology).
  4. Engel P, Wagner N, Tedder TF. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1995:667-679.
  5. Mason DY, Cordell JL, Tse AG, et al. The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991; 147(11):2474-2482. (Biology). View Reference
  6. Sakaguchi N, Kashiwamura S, Kimoto M, Thalmann P, Melchers F. B lymphocyte lineage-restricted expression of mb-1, a gene with CD3-like structural properties. EMBO J. 1988; 7(11):3457-3464. (Biology). View Reference
  7. Verschuren MC, Comans-Bitter WM, Kapteijn CA, et al. Transcription and protein expression of mb-1 and B29 genes in human hematopoietic malignancies and cell lines. Leukemia. 1993; 7:1939-1947. (Biology).
View All (7) View Less
341643 Rev. 1

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