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PE Mouse Anti-Human TCRγ/δ
Product Details
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BD™
TCRgd; γδ TCR; TRG@/TRD@; TCRG/TCRD; TCR gamma delta
Human
Mouse BALB/c IgG1
Sepharose® bead/CD3/γ/δ TCR complex
Flow cytometry
25 μg/mL
20 μL
6964, 6965
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347907 Rev. 1
Antibody Details
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11F2

The Anti–TCRγ/δ-1 antibody, clone 11F2, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with a Sepharose®  bead/CD3/γ/δ TCR complex.

The Anti–TCRγ/δ-1 antibody reacts with a framework epitope of the γ/δ T-cell antigen receptor (TCR). The γ/δ TCR is a heterodimeric glycoprotein that is noncovalently associated with the CD3 antigen. The γ and δ TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. The γ chain forms either disulfide-linked or non–disulfide-linked heterodimers with the δ-subunit.

347907 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
347907 Rev.1
Citations & References
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View product citations for antibody "347907" on CiteAb

Development References (16)

  1. Blink SE, Miller SD. The contribution of gammadelta T cells to the pathogenesis of EAE and MS.. Curr Mol Med. 2009; 9(1):15-22. (Biology). View Reference
  2. Bonneville M, O'Brien RL, Born WK. Gammadelta T cell effector functions: a blend of innate programming and acquired plasticity. Nat Rev Immunol. 2110; 10(7):467-478. (Biology). View Reference
  3. Borst J, van Dongen JJ, Bolhuis RL, et al. Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody.. J Exp Med. 1988; 167(5):1625-44. (Biology). View Reference
  4. Cairo C, Hebbeler AM, Propp N, Bryant JL, Colizzi V, Pauza CD. Innate-like gammadelta T cell responses to mycobacterium Bacille Calmette-Guerin using the public V gamma 2 repertoire in Macaca fascicularis.. Tuberculosis (Edinb). 2007; 87(4):373-83. (Biology). View Reference
  5. Carding SR, Egan PJ. The importance of gamma delta T cells in the resolution of pathogen-induced inflammatory immune responses.. Immunol Rev. 2000; 173:98-108. (Biology). View Reference
  6. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  7. Chen ZW. Immune regulation of gammadelta T cell responses in mycobacterial infections.. Clin Immunol. 2005; 116(3):202-7. (Biology). View Reference
  8. Clinical and Laboratory Standards Institute. 2005. (Biology).
  9. García VE, Sieling PA, Gong J, et al. Single-cell cytokine analysis of gamma delta T cell responses to nonpeptide mycobacterial antigens.. J Immunol. 1997; 159(3):1328-35. (Biology). View Reference
  10. Huang D, Chen CY, Zhang M, et al. Clonal immune responses of Mycobacterium-specific γδ T cells in tuberculous and non-tuberculous tissues during M. tuberculosis infection.. PLoS ONE. 2012; 7(2):e30631. (Biology). View Reference
  11. Lanier L, Federspiel N, Ruitenberg J, et al. The T cell antigen receptor complex expressed on normal peripheral blood CD4-, CD8-T lymphocytes. J Exp Med. 1987; 165:1076-1094. (Biology).
  12. Lanier LL, Ruitenberg J, Bolhuis RL, Borst J, Phillips JH, Testi R. Structural and serological heterogeneity of gamma/delta T cell antigen receptor expression in thymus and peripheral blood.. Eur J Immunol. 1988; 18(12):1985-92. (Biology). View Reference
  13. Lanier LL, Serafini AT, Ruitenberg JJ, et al. The γ T-cell antigen receptor. J Clin Immunol. 1987; 7:429-440. (Biology).
  14. Testi R, Lanier LL. Functional expression of CD28 on T cell antigen receptor γ/δ-bearing T lymphocytes. Eur J Immunol. 1989; 19:185-188. (Biology).
  15. Urban EM, Chapoval AI, Pauza CD. Repertoire development and the control of cytotoxic/effector function in human γδ T cells. Clin Dev Immunol. 2010; 732893. (Biology).
  16. Voogt PJ, Falkenburg JH, Fibbe WE, et al. Normal hematopoietic progenitor cells and malignant lymphohematopoietic cells show different susceptibility to direct cell-mediated MHC-non-restricted lysis by T cell receptor-/CD3-, T cell receptor γ/δ+/CD3+ and T cell receptor-&α/β+/CD3+ lymphocytes. J Immunol. 1989; 142:1774-1780. (Biology).
View All (16) View Less
347907 Rev. 1

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