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PE Mouse Anti-Human HER-2/neu
Product Details
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BD™
HER2; NEU; ERBB2; p185HER2; erb-b2 receptor tyrosine kinase 2; CD340
Human
Mouse BALB/c IgG1
SKBR3 human breast carcinoma cells
Flow cytometry
20 μL
2064
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

The FITC and PE conjugates are each supplied in 1.0 mL of PBS. The APC conjugate is supplied in 0.5 mL. Please refer to the vial label for antibody concentration. PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340552 Rev. 1
Antibody Details
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NEU 24.7

Anti–HER-2/neu, clone Neu 24.7, is derived from the fusion of NSO myeloma cells with spleen cells from BALB/c mice injected with living SKBR3 human breast carcinoma cells.

Anti–HER-2/neu recognizes a single protein of 185 kdaltons from HER-2 transfected cells.

340552 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
340552 Rev.1
Citations & References
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View product citations for antibody "340552" on CiteAb

Development References (5)

  1. Fendly BM, Kotts C, Vetterlein D, et al. The extracellular domain of HER2/neu is a potential immunogen for active specific immunotherapy of breast cancer. J Biol Response Mod. 1990; 9(5):449-455. (Biology). View Reference
  2. Gullick WJ. The role of the epidermal growth factor receptor and the c-erbB-2 protein in breast cancer. Int J Cancer. 1990; 5:55-61. (Biology). View Reference
  3. Shalaby MR, Shepard HM, Presta L, et al. Development of humanized bispecific antibodies reactive with cytotoxic lymphocytes and tumor cells overexpressing the HER2 protooncogene.. J Exp Med. 1992; 175(1):217-25. (Biology). View Reference
  4. Stancovski I, Hurwitz E, Leitner O, Ullrich A, Yarden Y, Sela M. Mechanistic aspects of the opposing effects of monoclonal antibodies to the ERBB2 receptor on tumor growth.. Proc Natl Acad Sci USA. 1991; 88(19):8691-5. (Biology). View Reference
  5. Szollosi J, Balazs M, Feuerstein BG, Benz CC, Waldman FM. ERBB-2 (HER2/neu) gene copy number, p185HER-2 overexpression, and intratumor heterogeneity in human breast cancer. Cancer Res. 1995; 55(22):5400-5407. (Biology). View Reference
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340552 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

 

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.