PE-Cy™7 Mouse Anti-Human CD33

BD™ PE-Cy™7 Mouse Anti-Human CD33

Name: PE-Cy™7 Mouse Anti-Human CD33
Brand: BD™
SKU: 333946

Clone P67.6

(RUO (GMP))

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Product Details

Brand: BD™

Alternative Name: Siglec-3; SIGLEC3; Sialic acid-binding Ig-like lectin 3; p67; gp67; My9

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: CD33-transfected FMY9S5 Cells

Application: Flow cytometry

Concentration: 25 μg/mL

Vol. Per Test: 5 μL

Workshop Number: IV M503

Entrez Gene ID: 945

RRID: AB_399961

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

The monoclonal antibody is supplied as 12 μg purified immunoglobulin in 2.0 mL (6 μg/mL) of phosphate-buffered saline (PBS). The FITC conjugate is supplied as 3 μg in 1.0 mL (3 μg/mL) of PBS. The PE conjugate is supplied as 24 μg in 2.0 mL (12 μg/mL) of PBS. The PerCP-Cy5.5 conjugate is supplied as 6.3 μg in 1.0 mL (6.3 μg/mL) of PBS. The PE-Cy7 conjugate is supplied as 12.5 μg in 0.5 mL (25 μg/mL) of PBS. The APC conjugate is supplied as 6.2 μg in 0.5 mL (12.4 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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Antibody Details

P67.6

The CD33 antibody, clone P67-6, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with FMY9S5 cells containing the CD33 gene.

The CD33 antibody recognizes a human myelomonocytic antigen, with a molecular weight of 67 kilodaltons (kDa).

333946 Rev. 1

Format Details

PE-Cy7

PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE-Cy7

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 781 nm

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Citations & References

Development References (7)

Andrews RG, Takahashi M, Segal GM, Powell JS, Bernstein ID, Singer JW. The L4F3 antigen is expressed by unipotent and multipotent colony-forming cells but not by their precursors. Blood. 1986; 68:1030-1035. (Biology).
Andrews RG, Torok-Storb B, Bernstein ID. Myeloid-associated differentiation antigens on stem cells and their progeny identified by monoclonal antibodies.. Blood. 1983; 62(1):124-32. (Biology). View Reference
Bernstein ID, Singer JW, Andrews RG, et al. Treatment of acute myeloid leukemia cells in vitro with a monoclonal antibody recognizing a myeloid differentiation antigen allows normal progenitor cells to be expressed.. J Clin Invest. 1987; 79(4):1153-9. (Biology). View Reference
Dinndorf PA, Andrews RG, Benjamin D, Ridgway D, Wolff L, Bernstein ID. Expression of normal myeloid-associated antigens by acute leukemia cells.. Blood. 1986; 67(4):1048-53. (Biology). View Reference
Foon KA, Todd RF. Immunologic classification of leukemia and lymphoma.. Blood. 1986; 68(1):1-31. (Biology). View Reference
Köller U, Peschel CH. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:812-813.
Terstappen LW, Hollander Z, Meiners H, Loken MR. Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J Leukoc Biol. 1990; 48(2):138-148. (Biology). View Reference

View All (7)

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