Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
India (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      FITC Mouse Anti-Human BrdU with DNase
      Product Details
      Down Arrow Up Arrow


      BD FastImmune™
      Human
      Mouse IgG1
      Iodouridine
      Intracellular staining (flow cytometry) , Flow cytometry
      10 μg/mL
      20 μL
      AB_400443
      Balanced salt solution with DNase, gelatin, and 0.1% sodium azide.
      RUO (GMP)


      Preparation And Storage

      Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

      Show More blue down arrow Show Less blue up arrow
      340649 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      B44

      The Anti-BrdU antibody, clone B44, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with iodouridine-conjugated ovalbumin.

      Bromodeoxyuridine (BrdU) is a uridine derivative that can be incorporated specifically into DNA in place of thymidine. Anti-BrdU identifies BrdU (but not thymidine) in single-stranded DNA, free BrdU, or BrdU coupled to a protein carrier. The antibody also reacts with iodouridine. DNase-I cleaves each strand of DNA at random and permits the binding of Anti-BrdU antibody to its antigen (BrdU).

      340649 Rev. 1
      Format Details
      Down Arrow Up Arrow
      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      340649 Rev.1
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "340649" on CiteAb

      Development References (9)

      1. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
      2. Clinical and Laboratory Standards Institute. 2005. (Biology).
      3. ElGhazali GEB, Paulie S, Andersson G, et al. Number of interleukin-4– and interferon- γ –secreting human T cells reactive with tetanus toxoid and the mycobacterial antigen PPD or phytohemagglutindistinct response profiles depending on the type of antigen used for activation. Eur J Immunol. 1993; 23:2740-2745. (Biology).
      4. Gratzner HG. Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science. 1982; 218:474. (Biology).
      5. Gray JW. Monoclonal antibodies against bromodeoxyuridine (special issue). Science. 1985; 6:501-673. (Biology).
      6. Mehta BA, Maino VC. Simultaneous detection of DNA synthesis and cytokine production in staphylococcal enterotoxin B activated CD4+ T lymphocytes by flow cytometry. J Immunol Methods. 1997; 208:49-59. (Biology).
      7. Murlidhar G, Koch S, Broome HE, Swain SL. TCR triggering of anergic CD4 T cells in murine AIDS induces apoptosis rather than cytokine synthesis and proliferation. J Immunol. 1996; 157:625-635. (Biology).
      8. Rosenberg ES, Billingsley JM, Caliendo AM, et al. Vigorous HIV-1–specific CD4 T cell responses associated with control of viremia. Science. 1997; 278:1447-1450. (Biology).
      9. Waldrop S, Pitcher C, Peterson D, Maino V, Picker L. Determination of antigen-specific memory/effector CD4 T cell frequencies by flow cytometry. J Clin Invest. 1997; 99:1739-1750. (Biology).
      View All (9) View Less
      340649 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.