BD FastImmune™ Antibody Cocktail (IFN-γ FITC, CD69 PE, CD4 PerCP-Cy™5.5, CD3 APC)
Description
Anti-Hu–IFN-γ, clone 25723.11, is derived from the hybridization of mouse P3X-63- Ag8.653 myeloma cells with lymph node cells from BALB/c mice immunized with recombinant human IFN-γ. CD69, clone L78, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen-directed cytotoxic T-lymphocyte (CTL) cell line. CD4, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immununized with human peripheral blood T lymphocytes. CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes. Anti-Human Interferon- γ (Anti-Hu–IFN-γ) recognizes a 20- to 25-kilodalton (kd) glycoprotein. CD69 recognizes a very early human activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28- and 32-kd chains that are held together by disulfide bridges. The CD4 antigen, Mr 55 kd, is present on T-helper/inducer lymphocytes and monocytes. CD3 reacts with the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kd. The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kd).
Preparation And Storage
Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
| Description | Clone | Isotype | EntrezGene ID |
|---|---|---|---|
| N/A | N/A | | IgG2b, ,IgG1, κ,IgG1, κ,IgG1, κ | N/A |
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