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      Anti-Human CD45 FITC/CD34 PE
      Product Details
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      BD™
      Human
      Flow cytometry
      RUO (GMP)
      Phosphate buffered saline with BSA and 0.1% sodium azide.


      Description

      The reagent is provided in 1 mL of phosphate-buffered saline (PBS) containing bovine serum albumin (BSA) and 0.1% sodium azide. The reagent contains FITC-labeled CD45, clone 2D1, and PE-labeled CD34, clone 8G12.

      The CD45/CD34 antigen recognizes a 180 to 220-kilodalton (kDa) human leucocyte antigen that is a member of the leucocyte common antigen (LCA) family. The CD45/CD34 antigen is present on all human leucocytes and is weakly expressed on hematopoietic progenitor cells. CD34 recognizes the human progenitor cell antigen (HPCA). The CD34 antigen is present on immature hematopoietic precursor cells and all hematopoietic colony-forming cells in bone marrow and blood, including unipotent and pluripotent progenitor cells.

      The CD45 and CD34 antibodies are each composed of mouse γ1 heavy chains and kappa light chains.

      BD TruCOUNT™ Tubes can be used with this reagent. Add the reagent and the blood sample directly to the BD TruCOUNT Tube to directly determine the absolute count of the cell population of interest. Simply divide the number of cellular events by the number of bead events, and then multiply by the bead concentration.

      Preparation And Storage

      Store the reagent vials upright at 2° to 8°C to maintain stability. Keep the vials dry and protected from light. Do not use after the expiration date shown on the label.

      Store BD TruCOUNT Tubes in the foil pouch at room temperature (20° to 25°C) and use within 1 hour after removal from the foil pouch. Reseal the foil pouch immediately after each use. The tubes are stable in the closed pouch for the period shown on the pouch label. Once the pouch has been opened, do not use the tubes beyond the expiration date indicated on the packaging.

      • Do not freeze the reagents or expose them to direct light during storage or during incubation with cells.

      • Do not use the reagents if you observe any changes in appearance. Precipitation or a change in color indicates instability or deterioration.

      • Examine the desiccant each time you open the foil pouch to use a BD TruCOUNT Tube. If the desiccant has turned from blue to lavender, discard the remaining tubes.

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      341071 Rev. 1
      Components
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      Description Clone Isotype EntrezGene ID
      CD45 FITC 2D1 IgG1, κ N/A
      CD34 PE 8G12 IgG1, κ N/A
      341071 Rev. 1
      Citations & References
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      Development References (10)

      1. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
      2. Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwartz JF, Shaper JH. Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol. Civin CI, Strauss LC; 133(1):157-165. (Biology). View Reference
      3. Civin CI, Trischmann TM, Fackler MJ, et al. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:818-825.
      4. Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:788-803.
      5. Jackson AL, Warner NL. Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
      6. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. Blood. 1987; 70(5):1316-1324. (Biology). View Reference
      7. Protection of Laboratory Workers from Occupationally Acquired Infections–Second Edition; Approved Guideline. NCCLS document M29-A. (Biology).
      8. Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
      9. Siena S, Bregni M, Brando B, et al. Flow cytometry for clinical estimation of circulating hematopoietic progenitors for autologous transplantation in cancer patients.. Blood. 1991; 77(2):400-9. (Biology). View Reference
      10. Terstappen LW, Huang S, Safford M, Lansdorp PM, Loken MR. Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34+CD38- progenitor cells. Blood. 1991; 77(6):1218-1227. (Biology). View Reference
      View All (10) View Less
      341071 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

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