Description
The CD14 antibody, clone MφP9, is derived from hybridization of Sp2/0 mouse myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood monocytes from a patient with rheumatoid arthritis. The CD64 antibody, clone MD22, is derived from fusion of P3X63Ag8 myeloma cells with splenocytes from BALB/c nu/nu mice immunized with FcγRI. The CD14 antibody recognizes a human monocyte/macrophage antigen, with a molecular weight of 55 kilodaltons (kDa). The CD64 antibody recognizes the 72-kDa human FcγRI glycoprotein that can bind monomeric IgG.
Preparation And Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
| Description | Clone | Isotype | EntrezGene ID |
|---|---|---|---|
| CD14 FITC | MΦP9 | IgG2b, κ | N/A |
| CD64 PE | MΦP9 | IgG2b, κ | N/A |
Development References (18)
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Bernstein ID, Self S. Joint report of the Myeloid Section of the Second International Workshop on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human Myeloid and Hematopoietic Cells. New York, NY: Springer-Verlag; 1986:1-25.
-
Buckle A-M, Hogg N. The effect of IFN-γ and colony-stimulating factors on the expression of neutrophil cell membrane receptors. J Immunol. 1989; 143:2295-2301. (Biology).
-
Dimitriu-Bona A, Burmester GR, Waters SJ, Winchester RJ. Human mononuclear phagocyte differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol. 1983; 130(1):145-152. (Biology). View Reference
-
Ernst LK, van de Winkel JGJ, Chiu IM, Anderson CL. Three genes for the human high affinity Fc receptor for IgG (Fc γ RI) encode four distinct transcription products. J Biol Chem. 1992; 267:15692-15700. (Biology).
-
Fanger NA, Wardwell K, Shen L, Tedder TF, Guyre PM. Type I (CD64) and type II (CD32) Fc gamma receptor-mediated phagocytosis by human blood dendritic cells.. J Immunol. 1996; 157(2):541-8. (Biology). View Reference
-
Goyert SM, Ferrero E. Biochemical analysis of myeloid antigens and cDNA expression of gp55 (CD14). In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:613-619.
-
Herrmann F, Komischke B, Odenwald E, Ludwig W. Use of monoclonal antibodies as a diagnostic tool in human leukemia, I: Acute myeloid leukemia and acute phase of chronic myeloid leukemia. Blut. 1983; 47:157-163. (Biology).
-
Jayaram Y, Hogg N. Surface expression of CD14 molecules on human neutrophils. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:796-797.
-
Kerst JM, van de Winkel JGJ, Evans AH, et al. Granulocyte colony-stimulating factor induces hFc γ RI (CD64 antigen)–positive neutrophils via an effect on myeloid precursor cells. Blood. 1993; 6:1457-1464. (Biology).
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Krasinskas AM, Wasik MA, Kamoun M, Schretzenmair R, Moore J, Salhany KE. The usefulness of CD64, other monocyte-associated antigens, and CD45 gating in the subclassification of acute myeloid leukemias with monocytic differentiation. Am J Clin Pathol. 1998; 110(6):797-805. (Biology).
-
Olweus J, Terstappen LW, Thompson PA, Lund-Johansen F. Expression and function of receptors for stem cell factor and erythropoietin during lineage commitment of human hematopoietic progenitor cells. Blood. 1996; 88:1594-1607. (Biology). View Reference
-
Perussia B, Dayton ET, Lazarus R, Fanning V, Trinchieri G. Immune interferon induces the receptor for monomeric IgG1 on human monocytic and myeloid cells. J Exp Med. 1983; 158:1092-1113. (Biology).
-
Petroni KC, Shen L, Guyre PM. Modulation of human polymorphonuclear leukocyte IgG Fc receptors and Fc receptor-mediated functions by IFN-γ and glucocorticoids. J Immunol. 1988; 140:3467-3472. (Biology).
-
Repp R, Valerius T, Sendler A, et al. Neutrophils express the high affinity receptor for IgG (FcγRI, CD64) after in vivo application of recombinant human granulocyte colony-stimulating factor. r Blood. 1991; 78:885-889. (Biology).
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Shen L, Guyre PM, Fanger MW Polymorphonuclear leukocyte function triggered through the high affinity Fc receptor for monomeric IgG. J Immunol. 1987; 534-548. (Biology).
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Valerius T, Repp R, de Wit TP, et al. Involvement of the high-affinity receptor for IgG (Fc γ RI; CD64) in enhanced tumor cell cytotoxicity of neutrophils during granulocyte colony-stimulating factor therapy. Blood. 1993; 82:931-939. (Biology).
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van de Winkel JG, Anderson CL. Biology of human immunoglobulin G Fc receptors.. J Leukoc Biol. 1991; 49(5):511-24. (Biology). View Reference
-
van de Winkel JG, Capel PJ. Human IgG Fc receptor heterogeneity: molecular aspects and clinical implications.. Immunol Today. 1993; 14(5):215-21. (Biology). View Reference
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