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CD56 PE
Product Details
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BD™
N-CAM; NCAM1; NCAM-1; Neural cell adhesion molecule 1; NKH1; MSK39
Human
Mouse BALB/c X C57BL/6 IgG1, κ
KG1a Cell Line
Flow cytometry
50 μg/mL
20 μL
V NK19
4684,916
Phosphate buffered saline with gelatin and 0.1% sodium azide.
ASR


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340685 Rev. 1
Antibody Details
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MY31

CD56, clone MY31, is derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from (B6 x BALB/c) F1 mice immunized with the KG1a cell line.

CD56 recognizes an antigen present on natural killer (NK) lymphocytes that is the 140-kdalton (kDa) isoform of the neural cell adhesion molecule (NCAM). The 140-kDa core protein is extensively glycosylated to give a mature antigen.

340685 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
340685 Rev.1
Citations & References
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View product citations for antibody "340685" on CiteAb

Development References (8)

  1. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  2. Clinical and Laboratory Standards Institute. 2005. (Biology).
  3. Edelman G. Cell adhesion molecules. Science. 1983; 219:450-457. (Biology).
  4. Hercend T, Griffin JD, Bensussan A, et al. Generation of monoclonal antibodies to a human natural killer clone. Characterization of two natural killer-associated antigens, NKH1A and NKH2, expressed on subsets of large granular lymphocytes.. J Clin Invest. 1985; 75(3):932-43. (Biology). View Reference
  5. Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ, Phillips JH. Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56). J Immunol. 1991; 146(12):4421-4426. (Biology). View Reference
  6. Lanier LL, Le AM, Civin CI, Loken MR, Phillips JH. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 1986; 136(12):4480-4486. (Biology). View Reference
  7. Lanier LL, Testi R, Bindl J, Phillips J. Identity of the Leu-19 (CD56) leucocyte differentiation antigen and neural-cell adhesion molecule. J Exp Med. 1989; 169:2233-2238. (Biology).
  8. Schubert J, Lanier LL, Schmidt RE. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:699-702.
View All (8) View Less
340685 Rev. 1

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