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CD3 FITC/CD8 PE/CD45 PerCP/CD4 APC

CD3 FITC/CD8 PE/CD45 PerCP/CD4 APC

(IVD)
Product Details
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BD Multitest™
Human
Flow cytometry
IVD
Buffered saline with 0.1% sodium azide.


Description

BD Multitest™ CD3 fluorescein isothiocyanate (FITC)/CD8 phycoerythrin (PE)/CD45 peridinin chlorophyll protein (PerCP)/CD4 allophycocyanin (APC) is a four-color direct immunofluorescence reagent for use with a suitably equipped flow cytometer to identify and determine the percentages and absolute counts of mature human T lymphocytes (CD3+), suppressor/cytotoxic (CD3+CD8+) T-lymphocyte subsets, and helper/inducer (CD3+CD4+) T-lymphocyte subsets in erythrocyte-lysed whole blood. When used with TruCOUNT™ Tubes, absolute counts of these populations can be enumerated from a single tube. BD Multitest reagent and TruCOUNT Tubes can be used with the FACS Loader.

Preparation And Storage

1. Store the reagent at 2–8°C. Do not use after the expiration date shown on the label.

2. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the reagent vial dry.

3. Store TruCOUNT Tubes in their original foil pouch at 2–25°C. To avoid potential condensation, open the pouch only after it has reached room temperature and carefully reseal the pouch immediately after removing a tube. Examine the desiccant each time you open the pouch. If the desiccant has turned from blue to lavender, discard the remaining tubes. Use tubes within 1 hour after removal from the foil pouch and do not use beyond the expiration date indicated on the packaging.

342417 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
CD4 APC SK3 IgG1, κ N/A
CD3 FITC SK7 IgG1, κ N/A
CD8 PE SK1 IgG1, κ N/A
CD45 PerCP 2D1 IgG1, κ N/A
342417 Rev. 1
Citations & References
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Development References (42)

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  6. Brenner MB, Groh V, Porcelli SA, et al. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. 1986:145-149.
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  35. Prince HE, Hirji K, Waldbeser LS, Plaeger-Marshall S, Kleinman S, Lanier LL. Influence of racial background on the distribution of T-cell subsets and Leu 11-positive lymphocytes in healthy blood donors. Diagn Immunol. 1985; 3(1):33-37. (Biology).
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View All (42) View Less
342417 Rev. 1

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