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CD3 FITC/CD16 + CD56 PE/CD45 PerCP/CD19 APC

CD3 FITC/CD16 + CD56 PE/CD45 PerCP/CD19 APC

(IVD)
Product Details
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BD Multitest™
Human
Flow cytometry
IVD
Buffered saline with 0.1% sodium azide.


Description

BD Multitest™ CD3 fluorescein isothiocyanate (FITC)/CD16 + CD56 phycoerythrin (PE)/CD45 peridinin chlorophyll protein (PerCP)/CD19 allophycocyanin (APC) is a four-color direct immunofluorescence reagent for use with a suitably equipped flow cytometer to identify and determine the percentages and absolute counts of mature human T (CD3+), natural killer (NK) (CD3-CD16+CD56+), and B (CD3-CD19+) lymphocyte in erythrocyte-lysed whole blood. When used with TruCOUNT™ Tubes, absolute counts of these populations can be enumerated from a single tube. BD Multitest reagent and TruCOUNT Tubes can be used with the FACS Loader.

Preparation And Storage

1. Store the reagent at 2–8°C. Do not use after the expiration date shown on the label.

2. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the reagent vial dry.

3. Store TruCOUNT Tubes in their original foil pouch at 2–25°C. To avoid potential condensation, open the pouch only after it has reached room temperature and carefully reseal the pouch immediately after removing a tube. Examine the desiccant each time you open the pouch. If the desiccant has turned from blue to lavender, discard the remaining tubes. Use tubes within 1 hour after removal from the foil pouch and do not use beyond the expiration date indicated on the packaging.

342416 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
CD19 APC SJ25C1 IgG1, κ N/A
CD3 FITC SK7 IgG1, κ N/A
CD16 (FcγRIII) PE B73.1 IgG1, κ N/A
CD56 (NCAM-1) PE NCAM16.2 IgG2b, κ N/A
CD45 PerCP 2D1 IgG1, κ N/A
342416 Rev. 1
Citations & References
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Development References (37)

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342416 Rev. 1

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