Skip to main content Skip to navigation
Anti-Human CD4 FITC/CD38 PE/CD3 PerCP/HLA-DR APC with BD Trucount™ Tubes

Anti-Human CD4 FITC/CD38 PE/CD3 PerCP/HLA-DR APC with BD Trucount™ Tubes

(IVD)
Product Details
Down Arrow Up Arrow


BD Multitest™
Human
Flow cytometry
IVD
PBS containing bovine serum albumin, and 0.1% sodium azide.


Description

The CD4 antibody, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes. The CD38 antibody, clone HB7, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with the BJAB cell line. The CD3 antibody, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes. The Anti–HLA-DR antibody, clone L243, is derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells from BALB/c mice immunized with the human lymphoblastoid B-cell line RPMI 8866.

CD4 recognizes an antigen that interacts with class II molecules of the major histocompatibility complex (MHC) and is the primary receptor for the human immunodeficiency virus. The cytoplasmic portion of the antigen is associated with the protein tyrosine kinase p56lck.The CD4 antigen can regulate the function of the CD3 antigen/Tcell antigen receptor (TCR) complex. CD4 also reacts with monocytes/macrophages that have an antigen density lower than that on helper/inducer T lymphocytes. CD38 recognizes an integral membrane glycoprotein, Mr 45 kDalton (kDa), with a protein core of 35 kDa. CD3 recognizes the epsilon chain of the CD3 antigen/TCR complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kDa. The antigen recognized by the CD3 antibody is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa). Anti–HLA-DR recognizes a human class II MHC antigen. The antigen is a transmembrane glycoprotein composed of α and β subunits that have molecular weights of 36 and 27 kDa, respectively. Anti–HLA-DR reacts with a nonpolymorphic HLA-DR epitope and does not cross-react with HLA-DQ or HLA-DP molecules.

Preparation And Storage

The MultiTEST reagent is supplied as a combination of CD4 FITC, CD38 PE, CD3 PerCP, and Anti–HLA-DR APC in 1.0 mL of PBS containing bovine serum albumin, and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

337087 Rev. 1
Components
Down Arrow Up Arrow
Description Clone Isotype EntrezGene ID
CD4 FITC SK3 IgG1, κ 920
HLA-DR APC 104D2 IgG1, 3815
CD38 PE HB7 IgG1, κ N/A
CD3 PerCP SK7 IgG1, κ N/A
337087 Rev. 1
Citations & References
Down Arrow Up Arrow

Development References (46)

  1. Benito J, Zabay J, Gil J, et al. Quantitative alterations of the functionally distinct subsets of CD4 and CD8 T lymphocytes in asymptomatic HIV infection: Changes in the expression of CD45RO, CD45RA, CD11b, CD38, HLA-DR, and CD35 antigens. J Acquir Immune Defic Syndr Hum Retrovirol. 1997; 14:128-135. (Biology).
  2. Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF, ed. Leucocyte Typing. New York, NY: Springer-Verlag; 1984:9-108.
  3. Bishop G, Hall B, Duggin G, Horvath J, Sheil A, Tiller D. Immunopathology of renal allograft rejection analyzed with monoclonal antibodies to mononuclear cell markers. Kidney Internat. 1986; 29:708-717. (Biology).
  4. Bogner J, Matuschke B, Hienrich B, Schreiber M, Nerl C, Goebel F. Expansion of activated T lymphocytes (CD3+HLA-DR+) detectable in early stages of HIV-1 infection. Klin Wochenschr. 1990; 68:393-396. (Biology).
  5. Brenner M, Groh V, Porcelli A, et al. Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:1049-1053.
  6. Brodsky FM. A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes.. Immunogenetics. 1984; 19(3):179-94. (Biology). View Reference
  7. Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988; 6:629. (Biology).
  8. Dalgleish A, Beverly P, Clapham P, Crawford D, Greaves M, Weiss R. The CD4 (T4) antigen is an essential component of the receptor for the AIDS virus. Nature. 1984; 312:763-767. (Biology).
  9. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, et al. Knapp W, Dörken B, Gilks W, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York: Oxford University Press; 1989:86.
  10. Edwards JA, Durant BM, Jones DB, Evans PR, Smith JL. Differential expression of HLA class II antigens in fetal human spleen: relationship of HLA-DP, DQ, and DR to immunoglobulin expression.. J Immunol. 1986; 137(2):490-7. (Biology). View Reference
  11. Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R. Studies of a human T lymphocyte antigen recognized by a monoclonal antibody.. Proc Natl Acad Sci USA. 1981; 78(3):1791-5. (Biology). View Reference
  12. Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology). View Reference
  13. Garson JA, Beverly PCL, Coakham HB, Harper EJ. Monoclonal antibodies against human T lymphocytes label Purkinje neurons of many species. Nature. 1982; 298:375. (Biology).
  14. Giorgi J, Hultin L. Lymphocyte subset alterations and immunophenotyping by flow cytometry in HIV disease. Clin Immunol Newslett. 1990; 10(4):55-61. (Biology).
  15. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
  16. Kan EA, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with anti-Leu-4 antibody. J Immunol. 1983; 131(2):536-539. (Biology). View Reference
  17. Knowles R. Reinherz E, Haynes B, Nadler L, Bernstein I, ed. Leukocyte Typing II: Human T Lymphocytes. 1986:259-288.
  18. Kurrle R. Knapp W, ed. Leucocyte Typing IV. New York, NY: Oxford University Press; 1989:290-293.
  19. Lampson LA, Levy R. Two populations of Ia-like molecules on a human B cell line.. J Immunol. 1980; 125(1):293-9. (Biology). View Reference
  20. Landay A, Ohlsson-Wilhelm B, Giorgi JV. Application of flow cytometry to the study of HIV infection. AIDS. 1990; 4(6):479-497. (Biology). View Reference
  21. Lanier LL, Allison JP, Phillips JH. Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation. J Immunol. 1986; 137(8):2501-2507. (Biology). View Reference
  22. Legendre C, Schiffrin A, Weitzner G, Colle E, Guttmann R. Legendre C, Schiffrin A, Weitzner G, Colle E, Guttmann R. Diabetes. 1988; 37:792-795. (Biology).
  23. Lenki R, Bratt G, Holmberg V, Muirhead K, Sandstrom E. Indicators of T-cell activation: Correlation between quantitative CD38 expression and soluble CD8 levels in asymptomatic HIV+ individuals and healthy controls. Cytometry. 1998; 33:115-122. (Biology).
  24. Levacher M, Tallet S, Dazza MC, Dournon E, Rouveix B, Pocidalo JJ. T activation marker evaluation in ARC patients treated with AZT. Comparison with CD4+ lymphocyte count in non-progressors and progressors towards AIDS.. Clin Exp Immunol. 1990; 81(2):177-82. (Biology). View Reference
  25. Maddon PJ, Dalgleish AG, McDougal JS, Clapham PR, Weiss RA, Axel R. The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain.. Cell. 1986; 47(3):333-48. (Biology). View Reference
  26. Nicholson J, Jones B, Hubbard M. CD4 Tlymphocyte determinations on whole blood specimens using a single-tube three-color assay. Cytometry. 1993; 14:598-605. (Biology).
  27. Pezzutto A, Behm F, Callard RE. Flow cytometry analysis of the B-cell blind panel: joint report. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:165-174.
  28. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
  29. Reinherz EL, Kung PC, Goldstein G, Levey RH, Schlossman SF. Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci U S A. 1980; 77(3):1588-1592. (Biology). View Reference
  30. Robbins PA, Evans EL, Ding AH, Warner NL, Brodsky FM. Monoclonal antibodies that distinguish between class II antigens (HLA-DP, DQ, and DR) in 14 haplotypes.. Hum Immunol. 1987; 18(4):301-13. (Biology). View Reference
  31. Rudd CE, Burgess KE, Barber EK, Schlossman SF. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:326-327.
  32. Salazar-Gonzalez JF, Moody DJ, Giorgi JV, Martinez-Maza O, Mitsuyasu RT, Fahey JL. Reduced ecto-5'-nucleotidase activity and enhanced OKT10 and HLA-DR expression on CD8 (T suppressor/cytotoxic) lymphocytes in the acquired immune deficiency syndrome: evidence of CD8 cell immaturity. J Immunol. 1985; 135(3):1778-1785. (Biology). View Reference
  33. Stites DP, Casavant CH, McHugh TM, et al. Flow cytometric analysis of lymphocyte phenotypes in AIDS using monoclonal antibodies and simultaneous dual immunofluorescence.. Clin Immunol Immunopathol. 1986; 38(2):161-77. (Biology). View Reference
  34. Tedder TF, Clement LT, Cooper MD. Discontinuous expression of a membrane antigen (HB-7) during B lymphocyte differentiation. Tissue Antigens. 1984; 24(3):140-149. (Biology). View Reference
  35. Tedder TF, Crain M, Kubagawa H, Clement L, et al. Evaluation of lymphocyte differentiation in primary and secondary immunodeficiency diseases. J Immunol. 1985; 135:1786-1791. (Biology).
  36. Terstappen LW, Hollander Z, Meiners H, Loken MR. Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J Leukoc Biol. 1990; 48(2):138-148. (Biology). View Reference
  37. Terstappen LW, Hollander Z, Meiners H, Loken MR. Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J Leukoc Biol. 1990; 48(2):138-148. (Biology). View Reference
  38. Terstappen LW, Huang S, Picker LJ. Flow cytometric assessment of human T-cell differentiation in thymus and bone marrow. Blood. 1992; 79(3):666-677. (Biology). View Reference
  39. Terstappen LW, Huang S, Safford M, Lansdorp PM, Loken MR. Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34+CD38- progenitor cells. Blood. 1991; 77(6):1218-1227. (Biology). View Reference
  40. Tomkinson BE, Wagner DK, Nelson DL, Sullivan JL. Activated lymphocytes during acute Epstein-Barr virus infection.. J Immunol. 1987; 139(11):3802-7. (Biology). View Reference
  41. Vanham G, Kestens L, Gigase P, et al. Evidence for circulating activated cytotoxic T cells in HIV-infected subjects before the onset of opportunistic infections. Clin Exp Immunol. 1990; 82:3-9. (Biology).
  42. Warnke R, Miller R, Grogan T, Pederson M, Dilley J, Levy R. Immunologic phenotype in 30 patients with diffuse large-cell lymphoma.. N Engl J Med. 1980; 303(6):293-300. (Biology). View Reference
  43. Warnke RA, Levy R. Detection of T and B cell antigens with hybridoma monoclonal antibodies: a biotinavidin-horseradish peroxidase method. J Histochem Cytochem. 1980; 28:771-776. (Biology).
  44. Wood GS, Warner NL, Warnke RA. Anti–Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage. J Immunol. 1983; 131(1):212-216. (Biology). View Reference
  45. Zipf TF, Fox RI, Dilley J, Levy R. Definition of the high-risk acute lymphoblastic leukemia patient by immunological phenotyping with monoclonal antibodies.. Cancer Res. 1981; 41(11 Pt 2):4786-9. (Biology). View Reference
  46. van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
View All (46) View Less
337087 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.